Ferredoxin—NAD(+) reductase explained

Ferredoxin-NAD+ reductase
Ec Number:	1.18.1.3
Cas Number:	39369-37-4
Go Code:	0008860

In enzymology, a ferredoxin–NAD⁺ reductase is an enzyme that catalyzes the chemical reaction:

reduced ferredoxin + NAD⁺

\rightleftharpoons oxidized ferredoxin + NADH + H⁺

Thus, the two substrates of this enzyme are reduced ferredoxin and NAD⁺, whereas its 3 products are oxidized ferredoxin, NADH, and H⁺. This enzyme participates in fatty acid metabolism.

This enzyme belongs to the family of oxidoreductases, specifically those acting on iron-sulfur proteins as donor with NAD+ or NADP+ as acceptor.

The systematic name of this enzyme is ferredoxin:NAD⁺ oxidoreductase. There are a variety of names in common use:

• ferredoxin–nicotinamide adenine dinucleotide reductase
• ferredoxin reductase
• NAD⁺-ferredoxin reductase
• ferredoxin–NAD⁺ reductase
• ferredoxin–linked NAD⁺ reductase
• ferredoxin–NAD reductase

When NAD molecule is in its reduced form, the enzyme is referred to as:

• NADH-ferredoxin oxidoreductase
• reduced nicotinamide adenine dinucleotide-ferredoxin
• NADH-ferredoxin reductase
• NADH flavodoxin oxidoreductase
• NADH₂-ferredoxin oxidoreductase

Other enzymes in the family include:

• NADH-ferredoxin NAP reductase (component of naphthalene dioxygenase multicomponent enzyme system)
• NADH-ferredoxin TOL reductase (component of toluene dioxygenase)

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .

References

• Jungermann K, Thauer RK, Leimenstoll G, Decker K . 1973 . Function of reduced pyridine nucleotide-ferredoxin oxidoreductases in saccharolytic Clostridia . Biochim. Biophys. Acta . 305 . 268 - 80 . 4147457 . 2 . 10.1016/0005-2728(73)90175-8.