Fatty-acid peroxygenase explained

Fatty-acid peroxygenase
Ec Number:	1.11.2.4

Fatty-acid peroxygenase (fatty acid hydroxylase (ambiguous), P450 peroxygenase, CYP152A1, P450BS, P450SPalpha) is an enzyme with systematic name fatty acid:hydroperoxide oxidoreductase (RH-hydroxylating).^{[1] [2] [3] [4] [5] [6] [7] [8]} This enzyme catalyses the following chemical reaction

fatty acid + H2O2

\rightleftharpoons 3- or 2-hydroxy fatty acid + H2O

Fatty-acid peroxygenase is a cytosolic heme-thiolate protein.

Notes and References

1. Matsunaga I, Yamada M, Kusunose E, Nishiuchi Y, Yano I, Ichihara K . Direct involvement of hydrogen peroxide in bacterial alpha-hydroxylation of fatty acid . FEBS Letters . 386 . 2–3 . 252–4 . May 1996 . 8647293 . 10.1016/0014-5793(96)00451-6 . free . 1996FEBSL.386..252M .
2. Matsunaga I, Yamada M, Kusunose E, Miki T, Ichihara K . Further characterization of hydrogen peroxide-dependent fatty acid alpha-hydroxylase from Sphingomonas paucimobilis . Journal of Biochemistry . 124 . 1 . 105–10 . July 1998 . 9644252 . 10.1093/oxfordjournals.jbchem.a022068 .
3. Matsunaga I, Ueda A, Fujiwara N, Sumimoto T, Ichihara K . Characterization of the ybdT gene product of Bacillus subtilis: novel fatty acid beta-hydroxylating cytochrome P450 . Lipids . 34 . 8 . 841–6 . August 1999 . 10529095 . 10.1007/s11745-999-0431-3 . 4017380 .
4. Imai Y, Matsunaga I, Kusunose E, Ichihara K . Unique heme environment at the putative distal region of hydrogen peroxide-dependent fatty acid alpha-hydroxylase from Sphingomonas paucimobilis (peroxygenase P450(SPalpha) . Journal of Biochemistry . 128 . 2 . 189–94 . August 2000 . 10920253 . 10.1093/oxfordjournals.jbchem.a022740 . free .
5. Matsunaga I, Yamada A, Lee DS, Obayashi E, Fujiwara N, Kobayashi K, Ogura H, Shiro Y . Enzymatic reaction of hydrogen peroxide-dependent peroxygenase cytochrome P450s: kinetic deuterium isotope effects and analyses by resonance Raman spectroscopy . Biochemistry . 41 . 6 . 1886–92 . February 2002 . 11827534 . 10.1021/bi011883p .
6. Lee DS, Yamada A, Sugimoto H, Matsunaga I, Ogura H, Ichihara K, Adachi S, Park SY, Shiro Y . Substrate recognition and molecular mechanism of fatty acid hydroxylation by cytochrome P450 from Bacillus subtilis. Crystallographic, spectroscopic, and mutational studies . The Journal of Biological Chemistry . 278 . 11 . 9761–7 . March 2003 . 12519760 . 10.1074/jbc.M211575200 . free .
7. Matsunaga I, Shiro Y . Peroxide-utilizing biocatalysts: structural and functional diversity of heme-containing enzymes . Current Opinion in Chemical Biology . 8 . 2 . 127–32 . April 2004 . 15062772 . 10.1016/j.cbpa.2004.01.001 .
8. Shoji O, Wiese C, Fujishiro T, Shirataki C, Wünsch B, Watanabe Y . Aromatic C-H bond hydroxylation by P450 peroxygenases: a facile colorimetric assay for monooxygenation activities of enzymes based on Russig's blue formation . Journal of Biological Inorganic Chemistry . 15 . 7 . 1109–15 . September 2010 . 20490877 . 10.1007/s00775-010-0671-9 . 23025367 .