Exodeoxyribonuclease III explained

Exodeoxyribonuclease III
Ec Number:	3.1.11.2
Cas Number:	9037-44-9

Exodeoxyribonuclease III (EC 3.1.11.2, Escherichia coli exonuclease III, E. coli exonuclease III, endoribonuclease III) is an enzyme^{[1] [2] [3]} that catalyses the following reaction

Exonucleolytic cleavage in the 3′- to 5′-direction to yield nucleoside 5′-phosphates

This enzyme has a preference for double-stranded DNA.

Notes and References

1. Lindahl T, Gally JA, Edelman GM . Properties of deoxyribonuclease 3 from mammalian tissues . The Journal of Biological Chemistry . 244 . 18 . 5014–9 . September 1969 . 10.1016/S0021-9258(18)94303-6 . free . 5824576 .
2. Richardson CC, Kornberg A . A deoxyribonucleic acid phosphatase-exonuclease from Escherichia coli. I. Purification of the enzyme and characterization of the phosphatase activity . The Journal of Biological Chemistry . 239 . 242–50 . January 1964 . 10.1016/S0021-9258(18)51774-9 . free . 14114850 .
3. Richardson CC, Lehman IR, Kornberg A . A deoxyribonucleic acid phosphatase-exonuclease from Escherichia coli. II. Characterization of the exonuclease activity . The Journal of Biological Chemistry . 239 . 251–8 . January 1964 . 10.1016/S0021-9258(18)51775-0 . free . 14114851 .