Exodeoxyribonuclease (lambda-induced) explained

Exodeoxyribonuclease (lambda-induced)
Ec Number:	3.1.11.3
Cas Number:	37367-70-7

Exodeoxyribonuclease (lambda-induced) (EC 3.1.11.3, lambda exonuclease, phage lambda-induced exonuclease, Escherichia coli exonuclease IV, E. coli exonuclease IV, exodeoxyribonuclease IV, exonuclease IV) is an exonuclease.^{[1] [2]} This enzyme catalyses the following chemical reaction

Exonucleolytic cleavage in the 5′- to 3′-direction to yield nucleoside 5′-phosphates

This enzyme has preference for double-stranded DNA (dsDNA). This means that it degrades a single strand of dsDNA, primarily any strand which has a phosphate at its 5' end.^[3]

Notes and References

1. Lindahl T, Gally JA, Edelman GM . Deoxyribonuclease IV: a new exonuclease from mammalian tissues . Proceedings of the National Academy of Sciences of the United States of America . 62 . 2 . 597–603 . February 1969 . 5256235 . 277851 . 10.1073/pnas.62.2.597 . free . 1969PNAS...62..597L .
2. Little JW . An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction . The Journal of Biological Chemistry . 242 . 4 . 679–86 . February 1967 . 10.1016/S0021-9258(18)96258-7 . 6017737 . free .
3. Web site: Lambda Exonuclease . Epicentre . 2014-05-26 . 2014-05-27 . https://web.archive.org/web/20140527220714/http://www.epibio.com/docs/default-source/protocols/lambda-exonuclease.pdf . dead .