Exo-1,4-beta-D-glucosaminidase explained
| Exo-1,4-beta-D-glucosaminidase |
| Ec Number: | 3.2.1.165 |
Exo-1,4-beta-D-glucosaminidase (CsxA, GlcNase, exochitosanase, GlmA, exo-beta-D-glucosaminidase, chitosan exo-1,4-beta-D-glucosaminidase) is an enzyme with systematic name chitosan exo-(1->4)-beta-D-glucosaminidase.![1] [2] [3] [4] [5] This enzyme catalyses the following chemical reaction
Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini
Chitosan is a partially or totally N-deacetylated chitin derivative that is found in the cell walls of some phytopathogenic fungi .
Notes and References
- Nanjo F, Katsumi R, Sakai K . Purification and characterization of an exo-beta-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis . The Journal of Biological Chemistry . 265 . 17 . 10088–94 . June 1990 . 2351651 .
- Nogawa M, Takahashi H, Kashiwagi A, Ohshima K, Okada H, Morikawa Y . Purification and Characterization of Exo-beta-d-Glucosaminidase from a Cellulolytic Fungus, Trichoderma reesei PC-3-7 . Applied and Environmental Microbiology . 64 . 3 . 890–5 . March 1998 . 16349528 . 106342 .
- Fukamizo T, Fleury A, Côté N, Mitsutomi M, Brzezinski R . Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism . Glycobiology . 16 . 11 . 1064–72 . November 2006 . 16877749 . 10.1093/glycob/cwl026 . free .
- Côté N, Fleury A, Dumont-Blanchette E, Fukamizo T, Mitsutomi M, Brzezinski R . Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases . The Biochemical Journal . 394 . Pt 3 . 675–86 . March 2006 . 16316314 . 1383717 . 10.1042/bj20051436 .
- Ike M, Isami K, Tanabe Y, Nogawa M, Ogasawara W, Okada H, Morikawa Y . Cloning and heterologous expression of the exo-beta-D-glucosaminidase-encoding gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7 . Applied Microbiology and Biotechnology . 72 . 4 . 687–95 . October 2006 . 16636831 . 10.1007/s00253-006-0320-y .
