Erythromycin 12 hydroxylase explained

Erythromycin 12 hydroxylase
Ec Number:	1.14.13.154

Erythromycin 12 hydroxylase (EryK) is an enzyme with systematic name erythromycin-D,NADPH:oxygen oxidoreductase (12-hydroxylating) .^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

erythromycin D + NADPH + H⁺ + O₂

\rightleftharpoons erythromycin C + NADP⁺ + H₂O

Erythromycin 12 hydroxylase is responsible for the C-12 hydroxylation of the macrolactone ring.

Notes and References

1. Lambalot RH, Cane DE, Aparicio JJ, Katz L . Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK . Biochemistry . 34 . 6 . 1858–66 . February 1995 . 7849045 . 10.1021/bi00006a006 .
2. Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B . Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate . The Journal of Biological Chemistry . 284 . 42 . 29170–9 . October 2009 . 19625248 . 2781461 . 10.1074/jbc.M109.003590 . free .
3. Montemiglio LC, Gianni S, Vallone B, Savino C . Azole drugs trap cytochrome P450 EryK in alternative conformational states . Biochemistry . 49 . 43 . 9199–206 . November 2010 . 20845962 . 10.1021/bi101062v .