Endothiapepsin Explained

Endothiapepsin
Ec Number:	3.4.23.22
Cas Number:	37205-60-0

Endothiapepsin (Endothia aspartic proteinase, Endothia acid proteinase, Endothia parasitica acid proteinase, Endothia parasitica aspartic proteinase) is an enzyme.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

Hydrolysis of proteins with specificity similar to that of pepsin A; prefers hydrophobic residues at P1 and P1', but does not cleave Ala14-Leu in the B chain of insulin or Z-Glu-Tyr. Clots milk

This enzyme is isolated from the ascomycete Endothia parasitica.

Notes and References

1. Blundell TL, Jenkins JA, Sewell BT, Pearl LH, Cooper JB, Tickle IJ, Veerapandian B, Wood SP . X-ray analyses of aspartic proteinases. The three-dimensional structure at 2.1 A resolution of endothiapepsin . Journal of Molecular Biology . 211 . 4 . 919–41 . February 1990 . 2179568 . 10.1016/0022-2836(90)90084-Y .
2. Hemmings AM, Foundling SI, Sibanda BL, Wood SP, Pearl LH, Blundell T . Energy calculations on aspartic proteinases: human renin, endothiapepsin and its complex with an angiotensinogen fragment analogue, H-142 . Biochemical Society Transactions . 13 . 6 . 1036–41 . December 1985 . 3912234 . 10.1042/bst0131036 .
3. Book: Protease of Endothia parasitica . Whitaker, J.R. . Protease of Endothia parasitica . Methods Enzymol. . 1970 . 19 . 436–445 . 10.1016/0076-6879(70)19032-x. 978-0-12-181881-4 .
4. Williams DC, Witaker JR, Caldwell PV . Hydrolysis of peptide bonds of the oxidized B-chain of insulin by Endothia parasitica protease . Archives of Biochemistry and Biophysics . 149 . 1 . 52–61 . March 1972 . 4552802 . 10.1016/0003-9861(72)90298-6 .
5. Barkholt V . Amino acid sequence of endothiapepsin. Complete primary structure of the aspartic protease from Endothia parasitica . European Journal of Biochemistry . 167 . 2 . 327–38 . September 1987 . 3305016 . 10.1111/j.1432-1033.1987.tb13340.x .
6. Cooper J, Foundling S, Hemmings A, Blundell T, Jones DM, Hallett A, Szelke M . The structure of a synthetic pepsin inhibitor complexed with endothiapepsin . European Journal of Biochemistry . 169 . 1 . 215–21 . November 1987 . 3119339 . 10.1111/j.1432-1033.1987.tb13600.x . free .