Enamidase Explained

enamidase
Ec Number:	3.5.2.18
Go Code:	0043792

In enzymology, an enamidase is an enzyme that catalyzes the chemical reaction

6-oxo-1,4,5,6-tetrahydronicotinate + 2 H₂O

\rightleftharpoons 2-formylglutarate + NH₃

Thus, the two substrates of this enzyme are 6-oxo-1,4,5,6-tetrahydronicotinate and H₂O, whereas its two products are 2-formylglutarate and NH₃.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is 6-oxo-1,4,5,6-tetrahydronicotinate amidohydrolase.

References

• Alhapel A, Darley DJ, Wagener N, Eckel E, Elsner N, Pierik AJ . 2006 . Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri . Proc. Natl. Acad. Sci. U.S.A. . 103 . 12341 - 6 . 16894175 . 10.1073/pnas.0601635103 . 33 . 1562527 . 2006PNAS..10312341A . free .