ELOC explained

Elongin C is a protein that in humans is encoded by the ELOC gene.^{[1] [2]}

Function

Elongin C is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.^[3]

Interactions

TCEB1 has been shown to interact with:

• TCEB2^{[4] [5] [6] [7]} and
• Von Hippel-Lindau tumor suppressor.^{[5] [7] [8] [9] [10] [11] [12]}

References

Further reading

• Aso T, Lane WS, Conaway JW, Conaway RC . Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II . Science . 269 . 5229 . 1439–43 . 1995 . 7660129 . 10.1126/science.7660129 . 1995Sci...269.1439A . 37649908 .
• Kibel A, Iliopoulos O, DeCaprio JA, Kaelin WG . Binding of the von Hippel-Lindau tumor suppressor protein to Elongin B and C . Science . 269 . 5229 . 1444–6 . 1995 . 7660130 . 10.1126/science.7660130 . 1995Sci...269.1444K . 25410108 .
• Bradsher JN, Jackson KW, Conaway RC, Conaway JW . RNA polymerase II transcription factor SIII. I. Identification, purification, and properties . J. Biol. Chem. . 268 . 34 . 25587–93 . 1994 . 10.1016/S0021-9258(19)74431-7 . 8244996 . free .
• Pause A, Lee S, Worrell RA, Chen DY, Burgess WH, Linehan WM, Klausner RD . The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins . Proc. Natl. Acad. Sci. U.S.A. . 94 . 6 . 2156–61 . 1997 . 9122164 . 20057 . 10.1073/pnas.94.6.2156 . free . 1997PNAS...94.2156P .
• Lonergan KM, Iliopoulos O, Ohh M, Kamura T, Conaway RC, Conaway JW, Kaelin WG . Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor suppressor protein requires binding to complexes containing elongins B/C and Cul2 . Mol. Cell. Biol. . 18 . 2 . 732–41 . 1998 . 9447969 . 108784 . 10.1128/mcb.18.2.732.
• Zhang JG, Farley A, Nicholson SE, Willson TA, Zugaro LM, Simpson RJ, Moritz RL, Cary D, Richardson R, Hausmann G, Kile BJ, Kent SB, Alexander WS, Metcalf D, Hilton DJ, Nicola NA, Baca M . The conserved SOCS box motif in suppressors of cytokine signaling binds to elongins B and C and may couple bound proteins to proteasomal degradation . Proc. Natl. Acad. Sci. U.S.A. . 96 . 5 . 2071–6 . 1999 . 10051596 . 26738 . 10.1073/pnas.96.5.2071 . 1999PNAS...96.2071Z . free .
• Stebbins CE, Kaelin WG, Pavletich NP . Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function . Science . 284 . 5413 . 455–61 . 1999 . 10205047 . 10.1126/science.284.5413.455 . 1999Sci...284..455S .
• Pause A, Peterson B, Schaffar G, Stearman R, Klausner RD . Studying interactions of four proteins in the yeast two-hybrid system: structural resemblance of the pVHL/elongin BC/hCUL-2 complex with the ubiquitin ligase complex SKP1/cullin/F-box protein . Proc. Natl. Acad. Sci. U.S.A. . 96 . 17 . 9533–8 . 1999 . 10449727 . 22243 . 10.1073/pnas.96.17.9533 . free . 1999PNAS...96.9533P .
• Iwai K, Yamanaka K, Kamura T, Minato N, Conaway RC, Conaway JW, Klausner RD, Pause A . Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex . Proc. Natl. Acad. Sci. U.S.A. . 96 . 22 . 12436–41 . 1999 . 10535940 . 22941 . 10.1073/pnas.96.22.12436 . free . 1999PNAS...9612436I .
• Ohh M, Takagi Y, Aso T, Stebbins CE, Pavletich NP, Zbar B, Conaway RC, Conaway JW, Kaelin WG . Synthetic peptides define critical contacts between elongin C, elongin B, and the von Hippel-Lindau protein . J. Clin. Invest. . 104 . 11 . 1583–91 . 2000 . 10587522 . 481054 . 10.1172/JCI8161 .
• Aso T, Yamazaki K, Amimoto K, Kuroiwa A, Higashi H, Matsuda Y, Kitajima S, Hatakeyama M . Identification and characterization of Elongin A2, a new member of the Elongin family of transcription elongation factors, specifically expressed in the testis . J. Biol. Chem. . 275 . 9 . 6546–52 . 2000 . 10692460 . 10.1074/jbc.275.9.6546 . free .
• Adryan B, Decker HJ, Papas TS, Hsu T . Tracheal development and the von Hippel-Lindau tumor suppressor homolog in Drosophila . Oncogene . 19 . 24 . 2803–11 . 2000 . 10851083 . 10.1038/sj.onc.1203611 . free .
• Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW . Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex . Proc. Natl. Acad. Sci. U.S.A. . 97 . 19 . 10430–5 . 2000 . 10973499 . 27041 . 10.1073/pnas.190332597 . free . 2000PNAS...9710430K .
• Aso T, Yamazaki K, Aigaki T, Kitajima S . Drosophila von Hippel-Lindau tumor suppressor complex possesses E3 ubiquitin ligase activity . Biochem. Biophys. Res. Commun. . 276 . 1 . 355–61 . 2000 . 11006129 . 10.1006/bbrc.2000.3451 .
• Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW . Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase . J. Biol. Chem. . 276 . 32 . 29748–53 . 2001 . 11384984 . 10.1074/jbc.M103093200 . free.
• Li Z, Na X, Wang D, Schoen SR, Messing EM, Wu G . Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein . J. Biol. Chem. . 277 . 7 . 4656–62 . 2002 . 11739384 . 10.1074/jbc.M108269200 . free .
• Yamazaki K, Guo L, Sugahara K, Zhang C, Enzan H, Nakabeppu Y, Kitajima S, Aso T . Identification and biochemical characterization of a novel transcription elongation factor, Elongin A3 . J. Biol. Chem. . 277 . 29 . 26444–51 . 2002 . 11994304 . 10.1074/jbc.M202859200 . free .
• Porkka K, Saramäki O, Tanner M, Visakorpi T . Amplification and overexpression of Elongin C gene discovered in prostate cancer by cDNA microarrays . Lab. Invest. . 82 . 5 . 629–37 . 2002 . 12004003 . 10.1038/labinvest.3780457 . free .

Notes and References

1. Garrett KP, Haque D, Conaway RC, Conaway JW . A human cDNA encoding the small subunit of RNA polymerase II transcription factor SIII . Gene . 150 . 2 . 413–4 . Feb 1995 . 7821821 . 10.1016/0378-1119(94)90467-7 .
2. Duan DR, Pause A, Burgess WH, Aso T, Chen DY, Garrett KP, Conaway RC, Conaway JW, Linehan WM, Klausner RD . Inhibition of transcription elongation by the VHL tumor suppressor protein . Science . 269 . 5229 . 1402–6 . Oct 1995 . 7660122 . 10.1126/science.7660122 . 1995Sci...269.1402D . 22334719 .
3. Web site: Entrez Gene: TCEB1 transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C).
4. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . Oct 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
5. Ohh M, Takagi Y, Aso T, Stebbins CE, Pavletich NP, Zbar B, Conaway RC, Conaway JW, Kaelin WG . Synthetic peptides define critical contacts between elongin C, elongin B, and the von Hippel-Lindau protein . J. Clin. Invest. . 104 . 11 . 1583–91 . Dec 1999 . 10587522 . 481054 . 10.1172/JCI8161 .
6. Krumm A, Groudine M . Tumor suppression and transcription elongation: the dire consequences of changing partners . Science . 269 . 5229 . 1400–1 . Sep 1995 . 7660121 . 10.1126/science.7660121. 1995Sci...269.1400K . 39758696 .
7. Li Z, Na X, Wang D, Schoen SR, Messing EM, Wu G . Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein . J. Biol. Chem. . 277 . 7 . 4656–62 . Feb 2002 . 11739384 . 10.1074/jbc.M108269200 . free .
8. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . Large-scale mapping of human protein-protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 89 . 17353931 . 1847948 . 10.1038/msb4100134 . 2007 .
9. Tsuchiya H, Iseda T, Hino O . Identification of a novel protein (VBP-1) binding to the von Hippel-Lindau (VHL) tumor suppressor gene product . Cancer Res. . 56 . 13 . 2881–5 . Jul 1996 . 8674032 .
10. Min JH, Yang H, Ivan M, Gertler F, Kaelin WG, Pavletich NP . Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling . Science . 296 . 5574 . 1886–9 . Jun 2002 . 12004076 . 10.1126/science.1073440 . 2002Sci...296.1886M . 19641938 . free .
11. Hacker KE, Lee CM, Rathmell WK . VHL type 2B mutations retain VBC complex form and function . PLOS ONE . 3 . 11 . e3801 . 19030229 . 2583047 . 10.1371/journal.pone.0003801 . Zhang . Baohong . 2008 . 2008PLoSO...3.3801H . free .
12. Kim BY, Kim H, Cho EJ, Youn HD . Nur77 upregulates HIF-alpha by inhibiting pVHL-mediated degradation . Exp. Mol. Med. . 40 . 1 . 71–83 . Feb 2008 . 18305400 . 2679322 . 10.3858/emm.2008.40.1.71 .