Diphthine—ammonia ligase explained

Diphthine—ammonia ligase
Ec Number:	6.3.1.14
Cas Number:	114514-33-9
Go Code:	0017178

In enzymology, a diphthine—ammonia ligase (diphthamide synthase, diphthamide synthetase) is an enzyme that catalyzes the chemical reaction

ATP + diphthine + NH₃

\rightleftharpoons ADP + phosphate + diphthamide

The 3 substrates of this enzyme are ATP, diphthine, and NH₃, whereas its 3 products are ADP, phosphate, and diphthamide.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is diphthine:ammonia ligase (ADP-forming). Other names in common use include diphthamide synthase, and diphthamide synthetase.

References

• Moehring JM, Moehring TJ . 1988 . The post-translational trimethylation of diphthamide studied in vitro . J. Biol. Chem. . 263 . 3840 - 4 . 3346227 . 8 .
• Moehring TJ, Moehring JM . 1987 . Mutant cultured cells used to study the synthesis of diphthamide . UCLA Symp. Mol. Cell. Biol. . New Series . 45 . 53 - 63 .