Dendritic cell-based cancer vaccine explained

The dendritic cell-based cancer vaccine is an innovation in therapeutic strategy for cancer patients.

Dendritic cells (DCs) are antigen presenting cells for the induction of antigen specific T cell response.[1] DC-based immunotherapy is safe and can promote antitumor immune responses and prolonged survival of cancer patients.[2]

Human DC subsets

Immature dendritic cells

Non-activated (immature) DCs are usually located in the peripheral non-lymphoid tissues and they can present self-antigens to T cells, that leads to immune tolerance either through T cell deletion or through the differentiation of regulatory T cells.[3]

Mature dendritic cells

Mature DCs have ability to present antigens in the lymphoid tissues, and to prime, activate, and expand immune effector cells with unique functions and cytokine profiles.[4]

Myeloid dendritic cells (cDCs)

Myeloid or conventional DCs (cDCs) are derived from myeloid progenitor cells in the bone marrow and are characterized by expression of CD11c.[5] cDCs can be subdivided into 3 groups: monocyte-derived DCs, CD1a- interstitial DCs, and CD1a+ Langerhans cells.[6]

Plasmacytoid dendritic cells (pDCs)

Plasmacytoid dendritic cells (pDCs) differentiate from lymphoid progenitor cells in the lymphoid tissues.[7] They express CD123 and product high levels of type I interferon.[8] pDCs also contribute to inflammatory responses in the steady state and in pathology. During inflammatory response, inflammatory DCs (iDCs) are generated from monocytes.[9]

Function of cancer therapeutic vaccines

The main goal of the therapeutic vaccines is to elicit cellular immunity.[10] They should prime naïve T cell, and induce transition from chronically activated non-protective CD8+ T cells to healthy CD8+ T cells that can produce cytotoxic T lymphocytes (CTLs), which recognize and eliminate cancer cells by recognizing specific antigens. This process also creates long-lived memory CD8+ T cells that will act to prevent relapse.[11] The most critical step in vaccination is the effective presentation of cancer antigens to T cells, and because of DCs are the most efficient antigen presenting cells, they are the promising option for improvement of therapeutic vaccines.[12]

Methods for exploiting dendritic cells in cancer therapeutic vaccines

DC-based immunotherapy approach can be employed in two ways:

Direct targeting/stimulating of the DCs in vivo to accentuate their anticancer phenotype

Many trials evaluating in vivo DC stimulation with synthetic peptides failed because of inability of effective stimulation of CD4+ cellular responses and stimulation of Th2 type cytokines.[13] The solution showing clinical responses was pre-treatment with single-dose cyclophosphamide as well as vaccination with tumor associated antigens (TAAs) and granulocyte macrophage colony stimulating factor (GM-CSF).[14]

Stimulation of the DCs ex vivo and infusing them back into the host for carrying out anticancer effector function

In this way, DCs’ precursors are isolated from the patient through leukapheresis and after maturation/stimulation of these precursors ex vivo, fully mature DCs are injected back into the patient.[15] There are different ways applied to generate cancer cells-specific DCs. We can used specific TAAs, tumor lysates, created DC-cancer cell fusions, electroporation/transfection of DCs with total cancer cell-mRNA or tumor derived exosomes (TDEs) by the stimulation. There is also the possibility of additional co-stimulating with cytokine “cocktails” to assure strong maturation.

Dendritic cell vaccine against brain tumor

The most well-known source of antigens used for vaccines in Glioblastoma (Aggressive type of brain tumor) investigations were whole tumor lysate, CMV antigen RNA and tumor associated peptides for instance EGFRvIII. The initial studies showed that patients developed immune responses as measured by Interferon-gamma expression in the peripheral blood, systemic cytokine responses, or CD8+ antigen specific T cell expansion. Clinical response rates were not as vigorous as the immune response rates. Overall survival (OS) and progression free survival (PFS) varied in different studies but were enhanced compared to historical controls.

Dendritic cell vaccine against COVID-19

Autologous dendritic cells previously loaded ex-vivo with SARS-CoV-2 spike protein. Subjects eligible for treatment will be those who at baseline, are not actively infected with SARS-CoV-2, have no evidence of prior infection with SARS-CoV-2 based on serologic testing, and give informed consent for a vaccination with AV-COVID-19. The patient population will include the elderly and others at higher risk for poor outcomes after COVID-19 infection. For this reason, individuals will not be excluded solely on the basis of age, body mass index, history of hypertension, diabetes, cancer, or autoimmune disease.

Sipuleucel-T

Sipuleucel-T is the first DCs- based cancer vaccine for men with asymptomatic or minimally symptomatic metastatic castration-resistant prostate cancer (CRPC), approved by the US Food and Drug Administration (FDA) .[16] [17] It is an active cellular immunotherapy, which involves obtaining antigen-presenting autologous dendritic cells from the patient following a leukapheresis procedure.[18] The cells are incubated ex vivo in the presence of a recombinant fusion protein PA2024 containing a prostate antigen, prostate acid phosphatase and GM-CSF, an immune-cell activator. The cells are then returned to the patient to generate an immune response.[19] [20]

Notes and References

  1. Stockwin. Luke H.. McGonagle. Dennis. Martin. Iain G.. Blair. G. Eric. April 2000. Dendritic cells: Immunological sentinels with a central role in health and disease. Immunology and Cell Biology. 78. 2. 91–102. 10.1046/j.1440-1711.2000.00888.x. 10762408. 0818-9641. 7159383.
  2. Datta. Jashodeep. Terhune. Julia H.. Lowenfeld. Lea. Cintolo. Jessica A.. Xu. Shuwen. Roses. Robert E.. Czerniecki. Brian J.. 2014-12-12. Optimizing Dendritic Cell-Based Approaches for Cancer Immunotherapy. The Yale Journal of Biology and Medicine. 87. 4. 491–518. 0044-0086. 4257036. 25506283.
  3. Mellman. Ira. 2013-09-01. Dendritic Cells: Master Regulators of the Immune Response. Cancer Immunology Research. 1. 3. 145–149. 10.1158/2326-6066.CIR-13-0102. 2326-6066. 24777676. free.
  4. Dalod. Marc. Chelbi. Rabie. Malissen. Bernard. Lawrence. Toby. 2014-05-16. Dendritic cell maturation: functional specialization through signaling specificity and transcriptional programming. The EMBO Journal. 33. 10. 1104–1116. 10.1002/embj.201488027. 0261-4189. 24737868. 4193918.
  5. Chistiakov. Dimitry A.. Sobenin. Igor A.. Orekhov. Alexander N.. Bobryshev. Yuri V.. 2015-06-01. Myeloid dendritic cells: Development, functions, and role in atherosclerotic inflammation. Immunobiology. 220. 6. 833–844. 10.1016/j.imbio.2014.12.010. 25595536.
  6. Merad. Miriam. Sathe. Priyanka. Helft. Julie. Miller. Jennifer. Mortha. Arthur. 2013. The Dendritic Cell Lineage: Ontogeny and Function of Dendritic Cells and Their Subsets in the Steady State and the Inflamed Setting. Annual Review of Immunology. 31. 563–604. 10.1146/annurev-immunol-020711-074950. 0732-0582. 3853342. 23516985.
  7. McKenna. Kelli. Beignon. Anne-Sophie. Bhardwaj. Nina. January 2005. Plasmacytoid Dendritic Cells: Linking Innate and Adaptive Immunity. 538703. Journal of Virology. 79. 1. 17–27. 10.1128/JVI.79.1.17-27.2005. 0022-538X. 15596797.
  8. Swiecki. Melissa. Colonna. Marco. August 2015. The multifaceted biology of plasmacytoid dendritic cells. Nature Reviews Immunology. 15. 8. 471–485. 10.1038/nri3865. 26160613. 1474-1733. 4808588.
  9. Chistiakov. Dimitry A.. Orekhov. Alexander N.. Sobenin. Igor A.. Bobryshev. Yuri V.. 2014-07-25. Plasmacytoid dendritic cells: development, functions, and role in atherosclerotic inflammation. Frontiers in Physiology. 5. 279. 10.3389/fphys.2014.00279. 1664-042X. 4110479. 25120492. free.
  10. Book: Guo. Chunqing. Manjili. Masoud H.. Subjeck. John R.. Sarkar. Devanand. Fisher. Paul B.. Wang. Xiang-Yang. 2013. Therapeutic Cancer Vaccines: Past, Present and Future. Advances in Cancer Research. 119. 421–475. 10.1016/B978-0-12-407190-2.00007-1. 0065-230X. 3721379. 23870514. 9780124071902.
  11. Palucka. Karolina. Banchereau. Jacques Banchereau. Jacques. 2013-07-25. Dendritic cell-based cancer therapeutic vaccines. Immunity. 39. 1. 38–48. 10.1016/j.immuni.2013.07.004. 1074-7613. 3788678. 23890062.
  12. Anguille. Sébastien. Smits. Evelien L. Lion. Eva. van Tendeloo. Viggo F. Berneman. Zwi N. 2014-06-01. Clinical use of dendritic cells for cancer therapy. The Lancet Oncology. 15. 7. e257–e267. 10.1016/S1470-2045(13)70585-0. 24872109.
  13. Rosenberg. Steven A.. Yang. James C.. Schwartzentruber. Douglas J.. Hwu. Patrick. Marincola. Francesco M.. Topalian. Suzanne L.. Restifo. Nicholas P.. Dudley. Mark E.. Schwarz. Susan L.. March 1998. Immunologic and therapeutic evaluation of a synthetic peptide vaccine for the treatment of patients with metastatic melanoma. Nature Medicine. 4. 3. 321–327. 1078-8956. 2064864. 9500606. 10.1038/nm0398-321.
  14. Dudek. Aleksandra M.. Martin. Shaun. Garg. Abhishek D.. Agostinis. Patrizia. 2013-12-11. Immature, Semi-Mature, and Fully Mature Dendritic Cells: Toward a DC-Cancer Cells Interface That Augments Anticancer Immunity. Frontiers in Immunology. 4. 438. 10.3389/fimmu.2013.00438. 1664-3224. 3858649. 24376443. free.
  15. Palucka. Karolina. Banchereau. Jacques. 2012-03-22. Cancer immunotherapy via dendritic cells. Nature Reviews. Cancer. 12. 4. 265–277. 10.1038/nrc3258. 1474-175X. 3433802. 22437871.
  16. Hammerstrom. Aimee E.. Cauley. Diana H.. Atkinson. Bradley J.. Sharma. Padmanee. August 2011. Cancer Immunotherapy: Sipuleucel-T and Beyond. Pharmacotherapy. 31. 8. 813–828. 10.1592/phco.31.8.813. 0277-0008. 4159742. 21923608.
  17. Anassi. Enock. Ndefo. Uche Anadu. April 2011. Sipuleucel-T (Provenge) Injection. Pharmacy and Therapeutics. 36. 4. 197–202. 1052-1372. 3086121. 21572775.
  18. Graff. Julie N. Chamberlain. Erin D. 2014-12-18. Sipuleucel-T in the treatment of prostate cancer: an evidence-based review of its place in therapy. Core Evidence. 10. 1–10. 10.2147/CE.S54712. 1555-1741. 4279604. 25565923 . free .
  19. Rini. Brian I.. Weinberg. Vivian. Fong. Lawrence. Conry. Shauna. Hershberg. Robert M.. Small. Eric J.. 2006-07-01. Combination immunotherapy with prostatic acid phosphatase pulsed antigen-presenting cells (provenge) plus bevacizumab in patients with serologic progression of prostate cancer after definitive local therapy. Cancer. 107. 1. 67–74. 10.1002/cncr.21956. 0008-543X. 16736512. 25676266. free.
  20. Eager. Robert. Nemunaitis. John. 2005-07-01. GM-CSF Gene-Transduced Tumor Vaccines. Molecular Therapy. 12. 1. 18–27. 10.1016/j.ymthe.2005.02.012. 15963916. free.

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