Deacetoxycephalosporin-C hydroxylase explained

Deacetoxycephalosporin-C hydroxylase
Ec Number:	1.14.11.26

In enzymology, a deacetoxycephalosporin-C hydroxylase is an enzyme that catalyzes the chemical reaction

deacetoxycephalosporin C + 2-oxoglutarate + O₂

\rightleftharpoons deacetylcephalosporin C + succinate + CO₂

The 3 substrates of this enzyme are deacetoxycephalosporin C, 2-oxoglutarate, and O₂, whereas its 3 products are deacetylcephalosporin C, succinate, and CO₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with 2-oxoglutarate as one donor, and incorporation of one atom o oxygen into each donor. The systematic name of this enzyme class is deacetoxycephalosporin-C,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating). Other names in common use include deacetylcephalosporin C synthase, 3'-methylcephem hydroxylase, DACS, DAOC hydroxylase, and deacetoxycephalosporin C hydroxylase. This enzyme participates in penicillin and cephalosporin biosynthesis.

References

• Dotzlaf JE, Yeh WK . 1987 . Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium . J. Bacteriol. . 169 . 1611 - 8 . 3558321 . 4 . 211989 .
• Baker BJ, Dotzlaf JE, Yeh WK . 1991 . Deacetoxycephalosporin C hydroxylase of Streptomyces clavuligerus Purification, characterization, bifunctionality, and evolutionary implication . J. Biol. Chem. . 266 . 5087 - 93 . 2002049 . 8 .
• Coque JJ, Enguita FJ, Cardoza RE, Martin JF, Liras P . 1996 . Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase . Appl. Microbiol. Biotechnol. . 44 . 605 - 9 . 8703431 . 10.1007/BF00172492 . 5 .
• Ghag SK, Brems DN, Hassell TC, Yeh WK . Refolding and purification of Cephalosporium acremonium deacetoxycephalosporin C synthetase/hydroxylase from granules of recombinant Escherichia coli . Biotechnol. Appl. Biochem. . 24 . 109 - 19 . 8865604 .
• Schofield CJ . 2004 . Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase . J. Biol. Chem. . 279 . 15420 - 6 . 14734549 . 10.1074/jbc.M313928200 . Lipscomb . SJ . Hewitson . KS . Hensgens . CM . Baldwin . JE . Schofield . CJ . 15 . free .
• Wu XB, Fan KQ, Wang QH, Yang KQ . 2005 . C-terminus mutations of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase with improved activity toward penicillin analogs . FEMS Microbiol. Lett. . 246 . 103 - 10 . 15869968 . 10.1016/j.femsle.2005.03.043 . 1 . free .
• AT, Kosalkova K . 1994 . Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins . Antonie van Leeuwenhoek . 65 . 227 - 43 . 7847890 . 10.1007/BF00871951 . Gutiérrez . S . Fernández . FJ . Velasco . J . Fierro . F . Marcos . AT . Kosalkova . K . 3 .