Cyanohydrin beta-glucosyltransferase explained
| cyanohydrin beta-glucosyltransferase |
| Ec Number: | 2.4.1.85 |
| Cas Number: | 55354-52-4 |
| Go Code: | 0047792 |
In enzymology, a cyanohydrin beta-glucosyltransferase is an enzyme that catalyzes the chemical reaction
UDP-D-glucose + (S)-4-hydroxymandelonitrile
UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside
Thus, the two substrates of this enzyme are UDP-D-glucose and (S)-4-hydroxymandelonitrile, whereas its two products are UDP and (S)-4-hydroxymandelonitrile beta-D-glucoside.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-D-glucose:(S)-4-hydroxymandelonitrile beta-D-glucosyltransferase. Other names in common use include uridine diphosphoglucose-p-hydroxymandelonitrile, glucosyltransferase, UDP-glucose-p-hydroxymandelonitrile glucosyltransferase, uridine diphosphoglucose-cyanohydrin glucosyltransferase, uridine diphosphoglucose:aldehyde cyanohydrin, beta-glucosyltransferase, UDP-glucose:(S)-4-hydroxymandelonitrile beta-D-glucosyltransferase, UGT85B1, and UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase. This enzyme participates in tyrosine metabolism and cyanoamino acid metabolism.
References
- Reay PF, Conn EE . Eric Conn (biochemist) . 1974 . The purification and properties of a uridine diphosphate glucose: aldehyde cyanohydrin beta-glucosyltransferase from sorghum seedlings . J. Biol. Chem. . 249 . 5826 - 30 . 4416442 . 18 . 10.1016/S0021-9258(20)79891-1 . free .
- Jones PR, Moller BL, Hoj PB . 1999 . The UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase that catalyzes the last step in synthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor. Isolation, cloning, heterologous expression, and substrate specificity . J. Biol. Chem. . 274 . 35483 - 91 . 10585420 . 10.1074/jbc.274.50.35483 . 50 . free .
- Moller BL . 2003 . The in vitro substrate regiospecificity of recombinant UGT85B1, the cyanohydrin glucosyltransferase from Sorghum bicolor . . 64 . 143 - 51 . 12946413 . 10.1016/S0031-9422(03)00261-9 . Kristensen . C . Tattersall . DB . Jones . PR . Olsen . CE . Bak . S . Møller . BL . 1 .
- Busk PK, Moller BL . 2002 . Dhurrin synthesis in sorghum is regulated at the transcriptional level and induced by nitrogen fertilization in older plants . Plant Physiol. . 129 . 1222 - 31 . 12114576 . 10.1104/pp.000687 . 3 . 166516 .
- BL, Bak S . 2005 . Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome . Proc. Natl. Acad. Sci. U.S.A. . 102 . 1779 - 84 . 15665094 . 10.1073/pnas.0409233102 . Morant . M . Olsen . CE . Ekstrøm . CT . Galbraith . DW . Møller . BL . Bak . S . 5 . 545087 . free .
