CUL2 explained

Cullin-2 is a protein that in humans is encoded by the CUL2 gene.^{[1] [2]}

Interactions

CUL2 has been shown to interact with:

• CAND1,^[3]
• DCUN1D1,^[4]
• RBX1,^{[5] [6]}
• SAP130,^[7]
• TCEB2^{[7] [8]} and
• Von Hippel-Lindau tumor suppressor.^{[7] [8] [9] [10] [11]}

Further reading

• Kibel A, Iliopoulos O, DeCaprio JA, Kaelin WG . Binding of the von Hippel-Lindau tumor suppressor protein to Elongin B and C . Science . 269 . 5229 . 1444–6 . 1995 . 7660130 . 10.1126/science.7660130 . 1995Sci...269.1444K . 25410108 .
• Pause A, Lee S, Worrell RA, Chen DY, Burgess WH, Linehan WM, Klausner RD . The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins . Proc. Natl. Acad. Sci. U.S.A. . 94 . 6 . 2156–61 . 1997 . 9122164 . 20057 . 10.1073/pnas.94.6.2156 . 1997PNAS...94.2156P . free .
• Lonergan KM, Iliopoulos O, Ohh M, Kamura T, Conaway RC, Conaway JW, Kaelin WG . Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor suppressor protein requires binding to complexes containing elongins B/C and Cul2 . Mol. Cell. Biol. . 18 . 2 . 732–41 . 1998 . 9447969 . 108784 . 10.1128/mcb.18.2.732.
• Wada H, Yeh ET, Kamitani T . Identification of NEDD8-conjugation site in human cullin-2 . Biochem. Biophys. Res. Commun. . 257 . 1 . 100–5 . 1999 . 10092517 . 10.1006/bbrc.1999.0339 .
• Ohta T, Michel JJ, Schottelius AJ, Xiong Y . ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity . Mol. Cell . 3 . 4 . 535–41 . 1999 . 10230407 . 10.1016/S1097-2765(00)80482-7 . 19371828 . free .
• Pause A, Peterson B, Schaffar G, Stearman R, Klausner RD . Studying interactions of four proteins in the yeast two-hybrid system: structural resemblance of the pVHL/elongin BC/hCUL-2 complex with the ubiquitin ligase complex SKP1/cullin/F-box protein . Proc. Natl. Acad. Sci. U.S.A. . 96 . 17 . 9533–8 . 1999 . 10449727 . 22243 . 10.1073/pnas.96.17.9533 . 1999PNAS...96.9533P . free .
• Iwai K, Yamanaka K, Kamura T, Minato N, Conaway RC, Conaway JW, Klausner RD, Pause A . Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex . Proc. Natl. Acad. Sci. U.S.A. . 96 . 22 . 12436–41 . 1999 . 10535940 . 22941 . 10.1073/pnas.96.22.12436 . 1999PNAS...9612436I . free .
• Hori T, Osaka F, Chiba T, Miyamoto C, Okabayashi K, Shimbara N, Kato S, Tanaka K . Covalent modification of all members of human cullin family proteins by NEDD8 . Oncogene . 18 . 48 . 6829–34 . 2000 . 10597293 . 10.1038/sj.onc.1203093 . free .
• Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW . Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex . Proc. Natl. Acad. Sci. U.S.A. . 97 . 19 . 10430–5 . 2000 . 10973499 . 27041 . 10.1073/pnas.190332597 . 2000PNAS...9710430K . free .
• Lyapina S, Cope G, Shevchenko A, Serino G, Tsuge T, Zhou C, Wolf DA, Wei N, Shevchenko A, Deshaies RJ . Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome . Science . 292 . 5520 . 1382–5 . 2001 . 11337588 . 10.1126/science.1059780 . 2001Sci...292.1382L . 14224920 .
• Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW . Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase . J. Biol. Chem. . 276 . 32 . 29748–53 . 2001 . 11384984 . 10.1074/jbc.M103093200 . free .
• Ohh M, Kim WY, Moslehi JJ, Chen Y, Chau V, Read MA, Kaelin WG . An intact NEDD8 pathway is required for Cullin-dependent ubiquitylation in mammalian cells . EMBO Rep. . 3 . 2 . 177–82 . 2002 . 11818338 . 1083969 . 10.1093/embo-reports/kvf028 .
• Min JH, Yang H, Ivan M, Gertler F, Kaelin WG, Pavletich NP . Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling . Science . 296 . 5574 . 1886–9 . 2002 . 12004076 . 10.1126/science.1073440 . 2002Sci...296.1886M . 19641938 . free .
• Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R, Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW, Conaway RC . Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase . Proc. Natl. Acad. Sci. U.S.A. . 99 . 16 . 10353–8 . 2002 . 12149480 . 124918 . 10.1073/pnas.162424199 . 2002PNAS...9910353B . free .
• Min KW, Hwang JW, Lee JS, Park Y, Tamura TA, Yoon JB . TIP120A associates with cullins and modulates ubiquitin ligase activity . J. Biol. Chem. . 278 . 18 . 15905–10 . 2003 . 12609982 . 10.1074/jbc.M213070200 . free .
• Yan Q, Kamura T, Cai Y, Jin J, Ivan M, Mushegian A, Conaway RC, Conaway JW . Identification of Elongin C and Skp1 sequences that determine Cullin selection . J. Biol. Chem. . 279 . 41 . 43019–26 . 2004 . 15280393 . 10.1074/jbc.M408018200 . free .

Notes and References

1. Kipreos ET, Lander LE, Wing JP, He WW, Hedgecock EM . cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family . Cell . 85 . 6 . 829–39 . Aug 1996 . 8681378 . 10.1016/S0092-8674(00)81267-2 . 15805562 . free .
2. Web site: Entrez Gene: CUL2 cullin 2.
3. Min KW, Hwang JW, Lee JS, Park Y, Tamura TA, Yoon JB . TIP120A associates with cullins and modulates ubiquitin ligase activity . J. Biol. Chem. . 278 . 18 . 15905–10 . May 2003 . 12609982 . 10.1074/jbc.M213070200 . free .
4. Kim AY, Bommeljé CC, Lee BE, Yonekawa Y, Choi L, Morris LG, Huang G, Kaufman A, Ryan RJ, Hao B, Ramanathan Y, Singh B . SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation . J. Biol. Chem. . 283 . 48 . 33211–20 . Nov 2008 . 18826954 . 2586271 . 10.1074/jbc.M804440200 . free .
5. Dias DC, Dolios G, Wang R, Pan ZQ . CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16601–6 . Dec 2002 . 12481031 . 139190 . 10.1073/pnas.252646399 . free .
6. Ohta T, Michel JJ, Schottelius AJ, Xiong Y . ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity . Mol. Cell . 3 . 4 . 535–41 . Apr 1999 . 10230407 . 10.1016/s1097-2765(00)80482-7. 19371828 . free .
7. Menon S, Tsuge T, Dohmae N, Takio K, Wei N . Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome . BMC Biochem. . 9 . 1 . 18173839 . 2265268 . 10.1186/1471-2091-9-1 . 2008 . free .
8. Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW . Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase . J. Biol. Chem. . 276 . 32 . 29748–53 . Aug 2001 . 11384984 . 10.1074/jbc.M103093200 . free .
9. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . Large-scale mapping of human protein-protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 89 . 17353931 . 1847948 . 10.1038/msb4100134 . 2007 .
10. Ohh M, Takagi Y, Aso T, Stebbins CE, Pavletich NP, Zbar B, Conaway RC, Conaway JW, Kaelin WG . Synthetic peptides define critical contacts between elongin C, elongin B, and the von Hippel-Lindau protein . J. Clin. Invest. . 104 . 11 . 1583–91 . Dec 1999 . 10587522 . 481054 . 10.1172/JCI8161 .
11. Hacker KE, Lee CM, Rathmell WK . VHL type 2B mutations retain VBC complex form and function . PLOS ONE . 3 . 11 . e3801 . 19030229 . 2583047 . 10.1371/journal.pone.0003801 . Zhang . Baohong . 2008 . 2008PLoSO...3.3801H . free .