Cucurbitacin Delta23-reductase explained

cucurbitacin delta23-reductase
Ec Number:	1.3.1.5
Cas Number:	37256-38-5
Go Code:	0047791

In enzymology, a cucurbitacin Delta23-reductase is an enzyme that catalyzes the chemical reaction

23,24-dihydrocucurbitacin + NAD(P)+

\rightleftharpoons cucurbitacin + NAD(P)H + H⁺

The 3 substrates of this enzyme are 23,24-dihydrocucurbitacin, NAD⁺, and NADP⁺, whereas its 4 products are cucurbitacin, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 23,24-dihydrocucurbitacin:NAD(P)+ Delta23-oxidoreductase. This enzyme is also called NAD(P)H: cucurbitacin B Delta23-oxidoreductase. It employs one cofactor, manganese.

References

• Schabort JC, Potgieter DJ . 1968 . Cucurbitacin B delta 23-reductase from Cucurbita maxima. II Cofactor requirements, enzyme kinetics, substrate specificity and other characteristics . Biochim. Biophys. Acta . 151 . 47 - 53 . 4384331 . 1 . 10.1016/0005-2744(68)90159-9 .
• Schabort JC, Potgieter DJ, de Villiers V . 1968 . Cucurbitacin B delta 23-reductase from Cucurbita maxima. I. Assay methods, isolation and purification . Biochim. Biophys. Acta . 151 . 33 - 46 . 5640163 . 1 . 10.1016/0005-2744(68)90158-7.