Scanning electron cryomicroscopy explained

Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber. The cooling is usually achieved with liquid nitrogen.^[1] CryoSEM of biological samples with a high moisture content can be done faster with fewer sample preparation steps than conventional SEM. In addition, the dehydration processes needed to prepare a biological sample for a conventional SEM chamber create numerous distortions in the tissue leading to structural artifacts during imaging.^{[2] [3] [4] [5]}

See also

• Electron microscopy
• Electron cryomicroscopy
• Transmission electron cryomicroscopy

Notes and References

1. Book: Terje . Techniques in Microscopy for Biomedical Applications . World Scientific Publishing Co Pte Ltd . 2006 . 978-9812564344 . 115.
2. Hickey. Cian D.. Sheehan. Jeremiah J.. Wilkinson. Martin G.. Auty. Mark A. E.. Growth and location of bacterial colonies within dairy foods using microscopy techniques: a review. Frontiers in Microbiology. 18 February 2015. 6. 99 . 10.3389/fmicb.2015.00099. 25741328 . 4332360 . free.
3. Book: Electron Microscopy of Plant Pathogens . 1991 . Springer Berlin Heidelberg . 978-3-642-75818-8 . Lesemann . Dietrich-Eckhardt . Berlin, Heidelberg . Mendgen . Kurt.
4. Book: Schatten. Heide. Scanning electron microscopy for the life sciences. 2013. Cambridge University Press. Cambridge. 9780521195997. Online-Ausg..
5. Book: Biological low voltage field emission scanning electron microscopy . 2007 . Springer . 9780387729725 . Schatten . Heide . New York . Pawley . James.