Creatininase Explained

Creatininase
Ec Number:	3.5.2.10
Cas Number:	9025-13-2
Go Code:	0047789

Symbol:	Creatininase
Creatininase
Pfam:	PF02633
Interpro:	IPR003785
Scop:	1v7z

In enzymology, a creatininase is an enzyme that catalyses the hydrolysis of creatinine to creatine, which can then be metabolised to urea and sarcosine by creatinase.

creatinine + H₂O

\rightleftharpoons creatine

Thus, the two substrates of this enzyme are creatinine and H₂O, whereas its product is creatine.

Creatininase is a member of the urease-related amidohydrolases,^[1] the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is creatinine amidohydrolase. This enzyme is also called creatinine hydrolase. This enzyme participates in arginine and proline metabolism.

Structural studies

Creatininase from Pseudomonas putida has a core structure consisting of 3-layers, alpha/beta/alpha.^[2]

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes,,, and .

References

• Tsuru D, Oka I, Yoshimoto T . 1976 . Creatinine decomposing enzymes in Pseudomonas putida . Agric. Biol. Chem. . 40 . 5 . 1011 - 1018 . 10.1271/bbb1961.40.1011. free .

Notes and References

1. Yamamoto K, Oka M, Kikuchi T, Emi S . Cloning of the creatinine amidohydrolase gene from Pseudomonas sp. PS-7 . Biosci. Biotechnol. Biochem. . 59 . 7 . 1331–1332 . 1995 . 7670196 . 10.1271/bbb.59.1331. free .
2. Yoshimoto T, Tanaka N, Kanada N, Inoue T, Nakajima Y, Haratake M, Nakamura KT, Xu Y, Ito K . Crystal structures of creatininase reveal the substrate binding site and provide an insight into the catalytic mechanism . J. Mol. Biol. . 337 . 2 . 399–416 . March 2004 . 15003455 . 10.1016/j.jmb.2004.01.022 .