Coenzyme F420 hydrogenase explained

coenzyme F420 hydrogenase
Ec Number:	1.12.98.1
Cas Number:	9027-05-8
Go Code:	0050454

In enzymology, a coenzyme F420 hydrogenase is an enzyme that catalyzes the chemical reaction

H₂ + coenzyme F₄₂₀

\rightleftharpoons reduced coenzyme F₄₂₀

Thus, the two substrates of this enzyme are H₂ and coenzyme F420, whereas its product is reduced coenzyme F420.

This enzyme belongs to the family of oxidoreductases, specifically those acting on hydrogen as donor with other, known, acceptors. The systematic name of this enzyme class is hydrogen:coenzyme F420 oxidoreductase. Other names in common use include 8-hydroxy-5-deazaflavin-reducing hydrogenase, F420-reducing hydrogenase, and coenzyme F420-dependent hydrogenase. This enzyme participates in folate biosynthesis and is a critical part of energy conservation in some methanogens such as Methanosarcina barkeri. It has 3 cofactors: iron, nickel, and deazaflavin.

References

• ((Adams MWW)), Mortenson LE, ((Chen J-S)) . 1981 . Hydrogenase . Biochim. Biophys. Acta . 594 . 105 - 176 . 10.1016/0304-4173(80)90007-5 .
• Yamazaki S . 1982 . A selenium-containing hydrogenase from Methanococcus vannielii Identification of the selenium moiety as a selenocysteine residue . J. Biol. Chem. . 257 . 7926 - 9 . 6211447 . 14 .
• Fox JA, Livingston DJ, Orme-Johnson WH, Walsh CT . 1987 . 8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization . Biochemistry . 26 . 4219 - 27 . 3663585 . 10.1021/bi00388a007 . 14 .
• Muth E, Morschel E, Klein A . 1987 . Purification and characterization of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from the archaebacterium Methanococcus voltae . Eur. J. Biochem. . 169 . 571 - 7 . 3121317 . 10.1111/j.1432-1033.1987.tb13647.x . 3 . free .
• Baron SF, Ferry JG . 1989 . Purification and properties of the membrane-associated coenzyme F420-reducing hydrogenase from Methanobacterium formicicum . J. Bacteriol. . 171 . 3846 - 53 . 2738024 . 7 . 210134 .