Coenzyme F420-1:gamma-L-glutamate ligase explained
| Coenzyme F420-1:γ-L-glutamate ligase |
| Ec Number: | 6.3.2.34 |
Coenzyme F420-1:γ-L-glutamate ligase (F420:gamma-glutamyl ligase, CofE-AF, MJ0768, CofE) is an enzyme with systematic name L-glutamate:coenzyme F420-1 ligase (GDP-forming).[1] [2] This enzyme catalyses the following chemical reaction
GTP + coenzyme F420-1 + L-glutamate
GDP + phosphate + coenzyme γ-F
420-2
In some instances, this enzyme is fused with a domain, so that the coenzyme F420-1 produced by the latter is quickly ligated to coenzyme F420-2. This enzyme is also able to serially add additional γ-glutamyl groups to F420-2, producing F420-3 and so on.[3]
Notes and References
- Li H, Graupner M, Xu H, White RH . CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii . Biochemistry . 42 . 32 . 9771–9778 . August 2003 . 12911320 . 10.1021/bi034779b .
- Nocek B, Evdokimova E, Proudfoot M, Kudritska M, Grochowski LL, White RH, Savchenko A, Yakunin AF, Edwards A, Joachimiak A . 6 . Structure of an amide bond forming F(420):gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases . Journal of Molecular Biology . 372 . 2 . 456–469 . September 2007 . 17669425 . 2678844 . 10.1016/j.jmb.2007.06.063 .
- Web site: Rhea- Annotated reactions database . www.rhea-db.org.
Choi KP, Bair TB, Bae YM, Daniels L . Use of transposon Tn5367 mutagenesis and a nitroimidazopyran-based selection system to demonstrate a requirement for fbiA and fbiB in coenzyme F(420) biosynthesis by Mycobacterium bovis BCG . Journal of Bacteriology . 183 . 24 . 7058–7066 . December 2001 . 11717263 . 10.1128/JB.183.24.7058-7066.2001 . 95553 .
