Coenzyme F420-0:L-glutamate ligase explained

Coenzyme F420-0:L-glutamate ligase
Ec Number:	6.3.2.31

Coenzyme F₄₂₀-0:L-glutamate ligase (CofE-AF, MJ0768, CofE) is an enzyme with systematic name L-glutamate:coenzyme F420-0 ligase (GDP-forming).^{[1] [2]} This enzyme catalyses the following chemical reaction

\rightleftharpoons GDP + phosphate + coenzyme F420-1

In some instances, this enzyme is fused with a domain, so that the produced coenzyme F420-1 is quickly ligated to coenzyme F420-2.

Notes and References

1. Li H, Graupner M, Xu H, White RH . CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii . Biochemistry . 42 . 32 . 9771–8 . August 2003 . 12911320 . 10.1021/bi034779b .
2. Nocek B, Evdokimova E, Proudfoot M, Kudritska M, Grochowski LL, White RH, Savchenko A, Yakunin AF, Edwards A, Joachimiak A . Structure of an amide bond forming F(420):gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases . Journal of Molecular Biology . 372 . 2 . 456–69 . September 2007 . 17669425 . 2678844 . 10.1016/j.jmb.2007.06.063 .