Cobyrinate a,c-diamide synthase explained

Cobyrinate a,c-diamide synthase
Ec Number:	6.3.5.11

Cobyrinate a,c-diamide synthase, cobyrinic acid a,c-diamide synthetase, CbiA (gene)) is an enzyme which catalyses the chemical reaction^[1]

2 ATP + cobyrinate + 2 L-glutamine + 2 H₂O

\rightleftharpoons 2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate (overall reaction)

(1a) ATP + cobyrinate + L-glutamine + H₂O

\rightleftharpoons ADP + phosphate + cobyrinate c-monamide + L-glutamate

(1b) ATP + cobyrinate c-monamide + L-glutamine + H₂O

\rightleftharpoons ADP + phosphate + cobyrinate a,c-diamide + L-glutamate

This enzyme is a glutamine amidotransferase, part of the biosynthetic pathway to cobalamin (vitamin B₁₂) in anaerobic bacteria such as Salmonella typhimurium and Bacillus megaterium.

See also

• Cobalamin biosynthesis

Notes and References

1. Fresquet V, Williams L, Raushel FM . Mechanism of cobyrinic acid a,c-diamide synthetase from Salmonella typhimurium LT2 . Biochemistry . 43 . 33 . 10619–27 . August 2004 . 15311923 . 10.1021/bi048972x .