Cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate dehydrogenase explained

cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate dehydrogenase
Ec Number:	1.3.1.67
Go Code:	0018520

In enzymology, a cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate dehydrogenase is an enzyme that catalyzes the chemical reaction

cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD(P)+

\rightleftharpoons 4-methylcatechol + NAD(P)H + CO₂

The 3 substrates of this enzyme are cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate, NAD⁺, and NADP⁺, whereas its 4 products are 4-methylcatechol, NADH, NADPH, and CO₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate:NAD(P)+ oxidoreductase (decarboxylating). This enzyme participates in toluene and xylene degradation.

References

• Whited GM, McCombie WR, Kwart LD, Gibson DT . 1986 . Identification of cis-diols as intermediates in the oxidation of aromatic acids by a strain of Pseudomonas putida that contains a TOL plasmid . J. Bacteriol. . 166 . 1028 - 39 . 3711022 . 3 . 215228 .