Choloylglycine hydrolase explained

choloylglycine hydrolase
Ec Number:	3.5.1.24
Cas Number:	37289-07-9
Go Code:	0045302

In enzymology, a choloylglycine hydrolase is an enzyme that catalyzes the chemical reaction

3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H₂O

\rightleftharpoons 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

Thus, the two substrates of this enzyme are 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine and H₂O, whereas its two products are 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate and glycine.^{[1] [2]}

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine amidohydrolase. Other names in common use include glycocholase, bile salt hydrolase, and choloyltaurine hydrolase. This enzyme participates in bile acid biosynthesis.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes,,, and .

Research

A 2018 study has linked the enzyme in gut bacteria to obesity and carbohydrate metabolism dominating over fat metabolism.

References

• Nair PP, Gordon M, Reback J . 1967 . The enzymatic cleavage of the carbon-nitrogen bond in 3-alpha, 7-alpha, 12-alpha-trihydroxy-5-beta-cholan-24-oylglycine . J. Biol. Chem. . 242 . 7 - 11 . 6016335 . 1 . 10.1016/S0021-9258(18)96311-8 . free .
• Stellwag EJ, Hylemon PB . 1976 . Purification and characterization of bile salt hydrolase from Bacteroides fragilis subsp. fragilis . Biochim. Biophys. Acta . 452 . 165 - 76 . 10993 . 1 . 10.1016/0005-2744(76)90068-1.

Notes and References

1. Coleman JP, Hudson LL . Cloning and characterization of a conjugated bile acid hydrolase gene from Clostridium perfringens. . Appl Environ Microbiol . 1995 . 61 . 7 . 2514–20 . 7618863 . 10.1128/AEM.61.7.2514-2520.1995. 167523 . 1995ApEnM..61.2514C .
2. Rossocha M, Schultz-Heienbrok R, von Moeller H, Coleman JP, Saenger W . Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product. . Biochemistry . 2005 . 44 . 15 . 5739–48 . 10.1021/bi0473206 . 15823032 .