Cholate—CoA ligase explained

Cholate—CoA ligase
Ec Number:	6.2.1.7
Cas Number:	9027-90-1

Cholate—CoA ligase (BAL, bile acid CoA ligase, bile acid coenzyme A ligase, choloyl-CoA synthetase, choloyl coenzyme A synthetase, cholic thiokinase, cholate thiokinase, cholic acid:CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl coenzyme A synthetase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA synthetase, THCA-CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate—CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate:CoA ligase (AMP-forming), cholyl-CoA synthetase, trihydroxycoprostanoyl-CoA synthetase) is an enzyme with systematic name cholate:CoA ligase (AMP-forming).^{[1] [2] [3] [4] [5] [6] [7]} This enzyme catalyses the following chemical reaction

(1) ATP + cholate + CoA

\rightleftharpoons AMP + diphosphate + choloyl-CoA

(2) ATP + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA

\rightleftharpoons AMP + diphosphate + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA

This enzyme requires Mg²⁺ for activity.

Notes and References

1. Elliott WH . The enzymic activation of cholic acid by guinea-pig-liver microsomes . The Biochemical Journal . 62 . 3 . 427–33 . March 1956 . 13303991 . 1215932 .
2. Elliott WH . The breakdown of adenosine triphosphate accompanying cholic acid activation by guinea-pig liver microsomes . The Biochemical Journal . 65 . 2 . 315–21 . February 1957 . 13403911 . 1199872 .
3. Prydz K, Kase BF, Björkhem I, Pedersen JI . Subcellular localization of 3 alpha, 7 alpha-dihydroxy- and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-coenzyme A ligase(s) in rat liver . Journal of Lipid Research . 29 . 8 . 997–1004 . August 1988 . 3183523 .
4. Schepers L, Casteels M, Verheyden K, Parmentier G, Asselberghs S, Eyssen HJ, Mannaerts GP . Subcellular distribution and characteristics of trihydroxycoprostanoyl-CoA synthetase in rat liver . The Biochemical Journal . 257 . 1 . 221–9 . January 1989 . 2521999 . 1135559 .
5. Mallonee DH, Adams JL, Hylemon PB . The bile acid-inducible baiB gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A ligase . Journal of Bacteriology . 174 . 7 . 2065–71 . April 1992 . 1551828 . 205821 .
6. Wheeler JB, Shaw DR, Barnes S . Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes . Archives of Biochemistry and Biophysics . 348 . 1 . 15–24 . December 1997 . 9390170 . 10.1006/abbi.1997.0391 .
7. Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S . Molecular cloning and expression of rat liver bile acid CoA ligase . Journal of Lipid Research . 43 . 12 . 2062–71 . December 2002 . 12454267 . 10.1194/jlr.M200260-JLR200 . free .