Volumetric pipette explained

A volumetric pipette, bulb pipette, or belly pipette [1] allows extremely accurate measurement (to four significant figures) of the volume of a solution.[2] It is calibrated to deliver accurately a fixed volume of liquid.

These pipettes have a large bulb with a long narrow portion above with a single graduation mark as it is calibrated for a single volume (like a volumetric flask). Typical volumes are 1, 2, 5, 10, 20, 25, 50 and 100 mL. Volumetric pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as to prepare solutions for titration.

ASTM standard E969 defines the standard tolerance for volumetric transfer pipettes. The tolerance depends on the size: a 0.5-mL pipette has a tolerance of ±0.006 mL, while a 50-mL pipette has a tolerance of ±0.05 mL. (These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.)

A specialized example of a volumetric pipette is the microfluid pipette (capable of dispensing as little as 10 μL) designed with a circulating liquid tip that generates a self-confining volume in front of its outlet channels.[3]

History

Pyrex started to make laboratory equipment in 1916 and became a favorite brand for the scientific community due to the borosilicate glass's natural properties. These included strength against; chemicals, thermal shift, and mechanical stress.[4]

External links

Notes and References

  1. Web site: Volumetric Pipette . Chemistry Glossary .
  2. Web site: Volumetric Pipettes . Labdepot, Inc .
  3. Ainla . A. . Jansson . E. T. . Stepanyants . N. . Orwar . O. . Jesorka . A. . A Microfluidic Pipette for Single-Cell Pharmacology . 10.1021/ac100480f . Analytical Chemistry . 82 . 11 . 4529–4536 . 2010 . 20443547.
  4. Web site: Volumetric Pipette . National Museum of American History .