Brachyurin Explained

Brachyurin
Ec Number:	3.4.21.32
Cas Number:	848900-32-3

Brachyurin (Uca pugilator collagenolytic proteinase, crab protease I, crab protease II) is an enzyme.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the Hydrolysis of proteins, with broad specificity for peptide bonds. Native collagen is cleaved about 75% of the length of the molecule from the N-terminus.

This enzyme is isolated from hepatopancreas of the fiddler crab, Uca pugilator.

Notes and References

1. Hurion N, Fromentin H, Keil B . Specificity of the collagenolytic enzyme from the fungus Entomophthora coronata: comparison with the bacterial collagenase from Achromobacter iophagus . Archives of Biochemistry and Biophysics . 192 . 2 . 438–45 . February 1979 . 219780 . 10.1016/0003-9861(79)90113-9 .
2. Book: Collagenolytic protease from fiddler crab (Uca pugilator) . Grant GA, Eisen AZ, Bradshaw RA . Collagenolytic protease from fiddler crab (Uca pugilator) . Methods Enzymol. . 1981 . 80 . 722–734 . 10.1016/s0076-6879(81)80055-9. 978-0-12-181980-4 .
3. Welgus HG, Grant GA, Jeffrey JJ, Eisen AZ . Substrate specificity of the collagenolytic serine protease from Uca pugilator: studies with collagenous substrates . Biochemistry . 21 . 21 . 5183–9 . October 1982 . 6756469 . 10.1021/bi00264a012 .
4. Welgus HG, Grant GA . Degradation of collagen substrates by a trypsin-like serine protease from the fiddler crab Uca pugilator . Biochemistry . 22 . 9 . 2228–33 . April 1983 . 6305411 . 10.1021/bi00278a026 .
5. Klimova OA, Borukhov SI, Solovyeva NI, Balaevskaya TO . The isolation and properties of collagenolytic proteases from crab hepatopancreas . Biochemical and Biophysical Research Communications . 166 . 3 . 1411–20 . February 1990 . 2154979 . 10.1016/0006-291x(90)91024-m .
6. Lu PJ, Liu HC, Tsai IH . The midgut trypsins of shrimp (Penaeus monodon). High efficiency toward native protein substrates including collagens . Biological Chemistry Hoppe-Seyler . 371 . 9 . 851–9 . September 1990 . 1963309 . 10.1515/bchm3.1990.371.2.851 .