Botryococcene C-methyltransferase explained

Botryococcene C-methyltransferase
Ec Number:	2.1.1.263

Botryococcene C-methyltransferase (TMT-3) is an enzyme with systematic name S-adenosyl-L-methionine:botryococcene C-methyltransferase.^[1]

Function

This enzyme catalyses the following chemical reaction:

\rightleftharpoons 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene (overall reaction)

(1a) S-adenosyl-L-methionine + C30 botryococcene

\rightleftharpoons S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene

(1b) S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene

\rightleftharpoons S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene

(2a) S-adenosyl-L-methionine + C30 botryococcene

\rightleftharpoons S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene

(2b) S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene

\rightleftharpoons S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene

This enzyme is isolated from the green alga Botryococcus braunii BOT22.

Notes and References

1. Niehaus TD, Kinison S, Okada S, Yeo YS, Bell SA, Cui P, Devarenne TP, Chappell J . Functional identification of triterpene methyltransferases from Botryococcus braunii race B . The Journal of Biological Chemistry . 287 . 11 . 8163–73 . March 2012 . 22241476 . 3318757 . 10.1074/jbc.m111.316059 . free .