Bis-tris methane explained
Bis-tris methane, also known as BIS-TRIS or BTM, is a buffering agent used in biochemistry. Bis-tris methane is an organic tertiary amine with labile protons having a pKa of 6.46 at 25 °C. It is an effective buffer between the pH 5.8 and 7.2. Bis-tris methane binds strongly to Cu and Pb ions as well as, weakly, to Mg, Ca, Mn, Co, Ni, Zn and Cd.[1]
See also
Notes and References
- Ferreira. Carlos M. H.. Pinto. Isabel S. S.. Soares. Eduardo V.. Soares. Helena M. V. M.. 2015. (Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review. RSC Advances. en. 5. 39. 30989–31003. 10.1039/c4ra15453c. 2046-2069. 10400.22/7404. free.
