Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) explained

bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
Ec Number:	3.6.1.17
Cas Number:	37289-29-5
Go Code:	0004081

In enzymology, a bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) is an enzyme that catalyzes the chemical reaction

P₁,P₄-bis(5'-guanosyl) tetraphosphate + H₂O

\rightleftharpoons GTP + GMP

Thus, the two substrates of this enzyme are P1,P4-bis(5'-guanosyl) tetraphosphate and H₂O, whereas its two products are GTP and GMP.

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is P1,P4-bis(5'-nucleosyl)-tetraphosphate nucleotidohydrolase. Other names in common use include bis(5'-guanosyl)-tetraphosphatase, bis(5'-adenosyl)-tetraphosphatase, diguanosinetetraphosphatase (asymmetrical), dinucleosidetetraphosphatase (asymmetrical), diadenosine P1,P4-tetraphosphatase, dinucleoside tetraphosphatase, and 1-P,4-P-bis(5'-nucleosyl)-tetraphosphate nucleotidohydrolase. This enzyme participates in purine metabolism and pyrimidine metabolism.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes,,,,,, and .

References

• Jakubowski H, Guranowski A . 1983 . Enzymes hydrolyzing ApppA and/or AppppA in higher plants Purification and some properties of diadenosine triphosphatase, diadenosine tetraphosphatase, and phosphodiesterase from yellow lupin (Lupinus luteus) seeds . J. Biol. Chem. . 258 . 9982 - 9 . 6309793 . 16 .
• Vallejo CG, Lobaton CD, Quintanilla M, Sillero A, Sillero MA . 1976 . Dinucleosidasetetraphosphatase in rat liver and Artemia salina . Biochim. Biophys. Acta . 438 . 304 - 9 . 181087 . 1 . 10.1016/0005-2744(76)90246-1.
• Warner AH, Finamore FJ . 1965 . Isolation, purification, and characterization of P1,P4-diguanosine 5'-tetraphosphate asymmetrical-pyrophosphohydrolase from brine shrimp eggs . Biochemistry . 4 . 1568 - 75 . 4955726 . 10.1021/bi00884a016 . 8 .