Biopterin Explained

Biopterins are pterin derivatives which function as endogenous enzyme cofactors in many species of animals and in some bacteria and fungi. The prototypical compound of the class is biopterin (6-(1,2-dihydroxypropyl)-pterin), as shown in the infobox. Biopterins act as cofactors for aromatic amino acid hydroxylases (AAAH), which are involved in synthesizing a number of neurotransmitters including dopamine, norepinephrine, epinepherine, and serotonin, along with several trace amines. Nitric oxide synthesis also uses biopterin derivatives as cofactors. In humans, tetrahydrobiopterin (BH4) is the endogenous cofactor for AAAH enzymes.

As with pterins in general, biopterins exhibit tautomerism. In other words, there are a number of forms that readily interconvert, differing by the placement of hydrogen atoms. Depictions of the chemical structure may therefore vary among sources.[1]

Compounds

Biopterin compounds found in the animal body include BH4, the free radical[2] BH3•, and the semi-oxidized form BH2. The fully oxidized form, i.e. "biopterin" proper, has little biological significance.

Bacteria produce several unique glycosides of biopterin (and of other pterins as well), using a specific BPt glucosyltransferase. They may have a function in UV protection.[3]

Biosynthesis

BH4 is the principal active cofactor. BH4 synthesis occurs through two principal pathways; the de novo pathway involves three enzymatic steps and proceeds from GTP, while the salvage pathway converts sepiapterin to BH4 using dihydrofolate reductase.[4] In addition, BH2 is recycled to BH4 by dihydrobiopterin reductase.

In the de novo pathway, GTP is converted to 7,8-dihydroneopterin triphosphate by GTP cyclohydrolase I (GTPCH-1, FolE), which expands the imidazole ring in GTP by one carbon. The intermediate is converted to 6-pyruvoyl-5,6,7,8-tetrahydropterin by 6-pyruvoyltetrahydropterin synthase, which removes the phosphate and produces the diketone (pyruvoyl) substituent. The final stage is mediated by sepiapterin reductase.[5]

Biopterin disorders

See main article: Tetrahydrobiopterin deficiency. A number of disorders of biopterin regulation exist.

Single-gene defects affecting the gene GCH1 block the first step in biopterin synthesis, and lead to dopamine-responsive dystonia, also known as Segawa's syndrome. This is due to the role of BH4 in synthesising neurotransmitters, including Dopamine, and is treated with supplementation with levodopa, which does not require BH4 for conversion to dopamine. GCH1 defects are autosomal dominant, meaning that only one defective gene copy is required for the condition to occur. Mouse gene knockout models that block biopterin synthesis completely die shortly after birth due to their inability to produce catecholamines and neurotransmitters.[6]

Biopterin synthesis disorders are also a cause of hyperphenylalaninemia; phenylalanine metabolism requires BH4 as a cofactor.[7]

In psychiatry, imbalances of biopterin concentrations have been hypothesized to be linked to mood disorders, particularly depression.[8]

External links

WiseGeek. (2012). What is biopterin?. Retrieved from http://www.wisegeek.com/what-is-biopterin.htm

Notes and References

  1. Benkovic . Stephen J. . Sammons . Douglas . Armarego . Wilfred L. F. . Waring . Paul . Inners . Ruth . Tautomeric nature of quinonoid 6,7-dimethyl-7,8-dihydro-6H-pterin in aqueous solution: a nitrogen-15 NMR study . Journal of the American Chemical Society . June 1985 . 107 . 12 . 3706–3712 . 10.1021/ja00298a048.
  2. 10.1074/jbc.M302227200 . 12692136 . Interactions of Peroxynitrite, Tetrahydrobiopterin, Ascorbic Acid, and Thiols: Implications For Uncoupling Endothelial Nitric-Oxide Synthase . Journal of Biological Chemistry . 278 . 25 . 22546–54 . 2003 . Kuzkaya . N. . Weissmann . N. . Harrison . D. G. . Dikalov . S. . free .
  3. Feirer . Nathan . Fuqua . Clay . Pterin function in bacteria . Pteridines . 1 May 2017 . 28 . 1 . 23–36 . 10.1515/pterid-2016-0012. 91132135 .
  4. 6572916. 393638. 1983. Nichol. C. A.. Biosynthesis of tetrahydrobiopterin by de novo and salvage pathways in adrenal medulla extracts, mammalian cell cultures, and rat brain in vivo. Proceedings of the National Academy of Sciences of the United States of America. 80. 6. 1546–50. Lee. C. L.. Edelstein. M. P.. Chao. J. Y.. Duch. D. S. . 10.1073/pnas.80.6.1546. 1983PNAS...80.1546N. free.
  5. 10.1042/BJ20110293. Tetrahydrobiopterin: Biochemistry and pathophysiology . 2011 . Werner . Ernst R. . Blau . Nenad . Thöny . Beat . Biochemical Journal . 438 . 3 . 397–414 . 21867484 .
  6. 12734191. 2003. Elzaouk. L. Dwarfism and low insulin-like growth factor-1 due to dopamine depletion in Pts-/- mice rescued by feeding neurotransmitter precursors and H4-biopterin. Journal of Biological Chemistry. 278. 30. 28303–11. Leimbacher. W. Turri. M. Ledermann. B. Burki. K. Blau. N. Thony. B. 10.1074/jbc.M303986200. free.
  7. Web site: Tetrahydrobiopterin . 2007-06-07 . dead . https://web.archive.org/web/20070629030636/http://www.uic.edu/classes/phar/phar332/Clinical_Cases/aa%20metab%20cases/PKU%20Cases/bioh4.htm . 2007-06-29 .
  8. Cavaleri et al. Blood concentrations of neopterin and biopterin in subjects with depression: A systematic review and meta-analysis Progress in Neuro-Psychopharmacology and Biological Psychiatry 2022. http://dx.doi.org/10.1016/j.pnpbp.2022.110633