Bicinchoninic acid explained

Bicinchoninic acid or BCA is a weak acid composed of two carboxylated quinoline rings. It is an organic compound with the formula (C9H5NCO2H)2. The molecule consists of a pair of quinoline rings, each bearing a carboxylic acid group. Its sodium salt forms a purple complex with cuprous ions.[1] [2] [3]

Bicinchoninic acid is most commonly employed in the bicinchoninic acid (BCA) assay, which is used to determine the total concentration of protein in a solution. Bicinchoninic acid is used to detect the presence of cuprous ions, due to its purple coloration via a biuret reaction. In this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.[4]

References

  1. 1985. Measurement of Protein Using Bicinchoninic Acid. Analytical Biochemistry. 150. 1. 76–85. 10.1016/0003-2697(85)90442-7. Smith . P.K. . Krohn . R.I. . Hermanson . G.T. . Mallia . A.K. . Gartner . F.H. . Provenzano . M.D. . Fujimoto . E.K. . Goeke . N.M. . Olson . B.J. . Klenk . D.C. . 3843705 .
  2. 10.1016/0003-2697(89)90101-2. Protein measurement using bicinchoninic acid: Elimination of interfering substances . 1989 . Brown . Rhoderick E. . Jarvis . Kari L. . Hyland . Kristi J. . Analytical Biochemistry . 180 . 1 . 136–139 . 2817336 .
  3. Book: 10.1016/S0076-6879(09)63008-1. Chapter 8 Quantitation of Protein . Guide to Protein Purification, 2nd Edition . Methods in Enzymology . 2009 . Noble . James E. . Bailey . Marc J.A. . 463 . 73–95 . 19892168 . 9780123745361 .
  4. http://www.piercenet.com/instructions/2161296.pdf Pierce BCA Protein Assay Kit

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