Bactericide Explained

A bactericide or bacteriocide, sometimes abbreviated Bcidal, is a substance which kills bacteria. Bactericides are disinfectants, antiseptics, or antibiotics.^[1] However, material surfaces can also have bactericidal properties based solely on their physical surface structure, as for example biomaterials like insect wings.

Disinfectants

The most used disinfectants are those applying

• active chlorine (i.e., hypochlorites, chloramines, dichloroisocyanurate and trichloroisocyanurate, wet chlorine, chlorine dioxide, etc.),
• active oxygen (peroxides, such as peracetic acid, potassium persulfate, sodium perborate, sodium percarbonate, and urea perhydrate),
• iodine (povidone-iodine, Lugol's solution, iodine tincture, iodinated nonionic surfactants),
• concentrated alcohols (mainly ethanol, 1-propanol, called also n-propanol and 2-propanol, called isopropanol and mixtures thereof; further, 2-phenoxyethanol and 1- and 2-phenoxypropanols are used),
• phenolic substances (such as phenol (also called "carbolic acid"), cresols such as thymol, halogenated (chlorinated, brominated) phenols, such as hexachlorophene, triclosan, trichlorophenol, tribromophenol, pentachlorophenol, salts and isomers thereof),
• cationic surfactants, such as some quaternary ammonium cations (such as benzalkonium chloride, cetyl trimethylammonium bromide or chloride, didecyldimethylammonium chloride, cetylpyridinium chloride, benzethonium chloride) and others, non-quaternary compounds, such as chlorhexidine, glucoprotamine, octenidine dihydrochloride etc.),
• strong oxidizers, such as ozone and permanganate solutions;
• heavy metals and their salts, such as colloidal silver, silver nitrate, mercury chloride, phenylmercury salts, copper sulfate, copper oxide-chloride etc. Heavy metals and their salts are the most toxic and environment-hazardous bactericides and therefore their use is strongly discouraged or prohibited
• strong acids (phosphoric, nitric, sulfuric, amidosulfuric, toluenesulfonic acids), pH < 1, and
• alkalis (sodium, potassium, calcium hydroxides), such as of pH > 13, particularly under elevated temperature (above 60 °C), kills bacteria.

Antiseptics

As antiseptics (i.e., germicide agents that can be used on human or animal body, skin, mucosae, wounds and the like), few of the above-mentioned disinfectants can be used, under proper conditions (mainly concentration, pH, temperature and toxicity toward humans and animals). Among them, some important are

• properly diluted chlorine preparations (f.e. Dakin's solution, 0.5% sodium or potassium hypochlorite solution, pH-adjusted to pH 7–8, or 0.5–1% solution of sodium benzenesulfochloramide (chloramine B)), some
• iodine preparations, such as iodopovidone in various galenics (ointment, solutions, wound plasters), in the past also Lugol's solution,
• peroxides such as urea perhydrate solutions and pH-buffered 0.1 – 0.25% peracetic acid solutions,
• alcohols with or without antiseptic additives, used mainly for skin antisepsis,
• weak organic acids such as sorbic acid, benzoic acid, lactic acid and salicylic acid
• some phenolic compounds, such as hexachlorophene, triclosan and Dibromol, and
• cationic surfactants, such as 0.05–0.5% benzalkonium, 0.5–4% chlorhexidine, 0.1–2% octenidine solutions.

Others are generally not applicable as safe antiseptics, either because of their corrosive or toxic nature.

Antibiotics

Bactericidal antibiotics kill bacteria; bacteriostatic antibiotics slow their growth or reproduction.

Bactericidal antibiotics that inhibit cell wall synthesis: the beta-lactam antibiotics (penicillin derivatives (penams), cephalosporins (cephems), monobactams, and carbapenems) and vancomycin.

Also bactericidal are daptomycin, fluoroquinolones, metronidazole, nitrofurantoin, co-trimoxazole, telithromycin.

Aminoglycosidic antibiotics are usually considered bactericidal, although they may be bacteriostatic with some organisms.

As of 2004, the distinction between bactericidal and bacteriostatic agents appeared to be clear according to the basic/clinical definition, but this only applies under strict laboratory conditions and it is important to distinguish microbiological and clinical definitions.^[2] The distinction is more arbitrary when agents are categorized in clinical situations. The supposed superiority of bactericidal agents over bacteriostatic agents is of little relevance when treating the vast majority of infections with gram-positive bacteria, particularly in patients with uncomplicated infections and noncompromised immune systems. Bacteriostatic agents have been effectively used for treatment that are considered to require bactericidal activity. Furthermore, some broad classes of antibacterial agents considered bacteriostatic can exhibit bactericidal activity against some bacteria on the basis of in vitro determination of MBC/MIC values. At high concentrations, bacteriostatic agents are often bactericidal against some susceptible organisms. The ultimate guide to treatment of any infection must be clinical outcome.

Surfaces

Material surfaces can exhibit bactericidal properties because of their crystallographic surface structure.

Somewhere in the mid-2000s it was shown that metallic nanoparticles can kill bacteria. The effect of a silver nanoparticle for example depends on its size with a preferential diameter of about 1 - 10 nm to interact with bacteria.^[3]

In 2013, cicada wings were found to have a selective anti-gram-negative bactericidal effect based on their physical surface structure.^[4] Mechanical deformation of the more or less rigid nanopillars found on the wing releases energy, striking and killing bacteria within minutes, hence called a mechano-bactericidal effect.^[5]

In 2020 researchers combined cationic polymer adsorption and femtosecond laser surface structuring to generate a bactericidal effect against both gram-positive Staphylococcus aureus and gram-negative Escherichia coli bacteria on borosilicate glass surfaces, providing a practical platform for the study of the bacteria-surface interaction.^[6]

See also

• List of antibiotics
• Microbicide
• Virucide

Notes and References

1. Clin Microbiol Rev. 1999. 12. 1. 147–179. 88911. Antiseptics and Disinfectants: Activity, Action, and Resistance. 9880479 . McDonnell . G . Russell . AD . 10.1128/cmr.12.1.147.
2. Clinical Relevance of Bacteriostatic versus Bactericidal Mechanisms of Action in the Treatment of Gram-Positive Bacterial Infections . Clin Infect Dis . 2004. 38. 6. 864–870. 10.1086/381972. 14999632 . Pankey . GA . Sabath . LD. free.
3. Morones . Jose Ruben . Elechiguerra . Jose Luis . Camacho . Alejandra . Holt . Katherine . Kouri . Juan B . Ramírez . Jose Tapia . Yacaman . Miguel Jose . 2005-10-01 . The bactericidal effect of silver nanoparticles . Nanotechnology . en . 16 . 10 . 2346–2353 . 10.1088/0957-4484/16/10/059 . 20818017 . 2005Nanot..16.2346R . 0957-4484.
4. Hasan . Jafar . Webb . Hayden K. . Truong . Vi Khanh . Pogodin . Sergey . Baulin . Vladimir A. . Watson . Gregory S. . Watson . Jolanta A. . Crawford . Russell J. . Ivanova . Elena P. . October 2013 . Selective bactericidal activity of nanopatterned superhydrophobic cicada Psaltoda claripennis wing surfaces . Applied Microbiology and Biotechnology . en . 97 . 20 . 9257–9262 . 10.1007/s00253-012-4628-5 . 23250225 . 16568909 . 0175-7598.
5. Ivanova . Elena P. . Linklater . Denver P. . Werner . Marco . Baulin . Vladimir A. . Xu . XiuMei . Vrancken . Nandi . Rubanov . Sergey . Hanssen . Eric . Wandiyanto . Jason . Truong . Vi Khanh . Elbourne . Aaron . 2020-06-09 . The multi-faceted mechano-bactericidal mechanism of nanostructured surfaces . Proceedings of the National Academy of Sciences . en . 117 . 23 . 12598–12605 . 10.1073/pnas.1916680117 . free . 0027-8424 . 32457154. 7293705 . 2020PNAS..11712598I .
6. Chen . C. . Enrico . A. . etal. Bactericidal surfaces prepared by femtosecond laser patterning and layer-by-layer polyelectrolyte coating . 10.1016/j.jcis.2020.04.107 . Journal of Colloid and Interface Science. 575 . 286–297 . 2020 . 32380320 . 2020JCIS..575..286C . free .