Aristolochene synthase explained
| aristolochene synthase |
| Ec Number: | 4.2.3.9 |
| Go Code: | 0045483 |
The enzyme aristolochene synthase (EC 4.2.3.9) catalyzes the chemical reaction
This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is (2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, aristolochene-forming). Other names in common use include sesquiterpene cyclase, trans,trans-farnesyl diphosphate aristolochene-lyase, trans,trans-farnesyl-diphosphate diphosphate-lyase (cyclizing and aristolochene-forming). This enzyme participates in terpenoid biosynthesis.
This protein may use the morpheein model of allosteric regulation.[1]
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and . They are both notable for the very high helix content of the structure.
References
- Cane DE, Prabhakaran PC, Oliver JS, McIlwaine DB . 1990 . Aristolochene biosynthesis. Stereochemistry of the deprotonation steps in the enzymatic cyclization of farnesyl pyrophosphate . J. Am. Chem. Soc. . 112 . 8 . 3209 - 3210 . 10.1021/ja00164a051 .
- Noguchi H, Rawlings BJ . 1989 . Aristolochene biosynthesis and enzymatic cyclization of farnesyl pyrophosphate . J. Am. Chem. Soc. . 111 . 24 . 8914 - 8916 . 10.1021/ja00206a022 .
- Hohn TM, Plattner RD . 1989 . Purification and characterization of the sesquiterpene cyclase aristolochene synthase from Penicillium roqueforti . Arch. Biochem. Biophys. . 272 . 137 - 43 . 2544140 . 10.1016/0003-9861(89)90204-X . 1 .
- Proctor RH, Hohn TM . 1993 . Aristolochene synthase. Isolation, characterization, and bacterial expression of a sesquiterpenoid biosynthetic gene (Ari1) from Penicillium roqueforti . J. Biol. Chem. . 268 . 4543 - 8 . 8440737 . 6 .
- Calvert MJ, Ashton PR, Allemann RK . 2002 . Germacrene A is a product of the aristolochene synthase-mediated conversion of farnesylpyrophosphate to aristolochene . J. Am. Chem. Soc. . 124 . 11636 - 41 . 12296728 . 10.1021/ja020762p . 39 .
Notes and References
- T. Selwood . E. K. Jaffe . amp . Dynamic dissociating homo-oligomers and the control of protein function. . Arch. Biochem. Biophys. . 519. 2. 131–43. 2011 . 22182754 . 10.1016/j.abb.2011.11.020 . 3298769.
