Α-Mannosidase Explained

α-Mannosidase (α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, 1,2-α-mannosidase, 1,2-α-D-mannosidase, exo-α-mannosidase) is an enzyme involved in the cleavage of the α form of mannose. Its systematic name is α-D-mannoside mannohydrolase.^{[1] [2]}

Isoenzymes

Humans express the following three α-mannosidase isoenzymes:

mannosidase, α, class 2B, member 1 Hgncid: 6826 Symbol: MAN2B1 Altsymbols: MANB Entrezgene: 4125 Omim: 609458 Refseq: NM_000528 Uniprot: O00754 Ecnumber: 3.2.1.24 Chromosome: 19 Locussupplementarydata: cen-q13.1

mannosidase, α, class 2B, member 2 Hgncid: 29623 Symbol: MAN2B2 Altsymbols: KIAA0935 Entrezgene: 23324 Refseq: NM_015274 Uniprot: Q9Y2E5 Ecnumber: 3.2.1.24 Chromosome: 4 Arm: p Band: 16.2

mannosidase, α, class 2C, member 1 Hgncid: 6827 Symbol: MAN2C1 Altsymbols: MANA1, MANA Entrezgene: 4123 Omim: 154580 Refseq: NM_006715 Uniprot: Q9NTJ4 Ecnumber: 3.2.1.24 Chromosome: 15 Arm: q Band: 11 Locussupplementarydata: -qter

Applications

It can be utilized in experiments that determine the effects of the presence or absence of mannose on specific molecules, such as recombinant proteins that are used in vaccine development.^[3]

Pathology

A deficiency can lead to α-mannosidosis.^[4]

External links

• GeneReviews/NCBI/NIH/UW entry on Alpha-Mannosidosis
• OMIM entries on Alpha-Mannosidosis

Notes and References

1. Presence of α-D-mannosidic linkage in glycoproteins. Liberation of D-mannose from various glycoproteins by α-mannosidase isolated from jack bean meal . Li, Y.-T. . J. Biol. Chem. . 1966 . 241 . 1010–1012 . 5905120 . 4. 10.1016/S0021-9258(18)96865-1 . free .
2. Role of α-D-mannosidases in the biosynthesis and catabolism of glycoproteins . Winchester, B. . Biochem. Soc. Trans. . 1984 . 12 . 522–524 . 6428944 . 3. 10.1042/bst0120522 .
3. Vlahopoulos S, Gritzapis AD, Perez SA, Cacoullos N, Papamichail M, Baxevanis CN . Mannose addition by yeast Pichia Pastoris on recombinant HER-2 protein inhibits recognition by the monoclonal antibody herceptin. . Vaccine . 2009 . 27 . 34 . 4704–8 . 19520203 . 10.1016/j.vaccine.2009.05.063 .
4. Malm D, Nilssen Ø . Alpha-mannosidosis. . Orphanet J Rare Dis . 2008 . 3 . 21 . 18651971 . 10.1186/1750-1172-3-21 . free . 2515294 .