Alpha-actinin-1 explained

Alpha-actinin-1 is a protein that in humans is encoded by the ACTN1 gene.^[1]

Function

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha-actinin-1 is an F-actin cross-linking protein – a bundling protein that is thought to anchor actin to a number of intracellular structures.^[2] Alpha-actinin-1 is a non-muscle cytoskeletal isoform found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.^[3]

Interactions

Alpha-actinin-1 has been shown to interact with:

• CDK5R1,^[4]
• CDK5R2,^[4]
• Collagen, type XVII, alpha 1,^[5]
• GIPC1,^[6]
• PDLIM1,^{[7] [8]}
• Protein kinase N1,^[9]
• SSX2IP,^[10] and
• Zyxin.^{[11] [12]}
• PTPRT (PTPrho)^[13]

See also

• Actinin

Further reading

• Snásel J, Pichová I . The cleavage of host cell proteins by HIV-1 protease. . Folia Biol. (Praha) . 42 . 5 . 227–30 . 1997 . 8997639 . 10.1007/BF02818986 . 7617882 .
• Menko S, Philp N, Veneziale B, Walker J . Integrins and development: how might these receptors regulate differentiation of the lens. . Ann. N. Y. Acad. Sci. . 842 . 36–41 . 1998 . 1 . 9599291 . 10.1111/j.1749-6632.1998.tb09629.x . 1998NYASA.842...36M . 31836557 .
• Yürüker B, Niggli V . Alpha-actinin and vinculin in human neutrophils: reorganization during adhesion and relation to the actin network. . J. Cell Sci. . 101 . 2. 403–14 . 1992 . 10.1242/jcs.101.2.403 . 1629252 .
• Tokuue Y, Goto S, Imamura M . Transfection of chicken skeletal muscle alpha-actinin cDNA into nonmuscle and myogenic cells: dimerization is not essential for alpha-actinin to bind to microfilaments. . Exp. Cell Res. . 197 . 2 . 158–67 . 1992 . 1720388 . 10.1016/0014-4827(91)90418-T . etal.
• Shoeman RL, Kesselmier C, Mothes E . Non-viral cellular substrates for human immunodeficiency virus type 1 protease. . FEBS Lett. . 278 . 2 . 199–203 . 1991 . 1991513 . 10.1016/0014-5793(91)80116-K . etal. free .
• Nishiyama M, Ozturk M, Frohlich M . Expression of human alpha-actinin in human hepatocellular carcinoma. . Cancer Res. . 50 . 19 . 6291–4 . 1990 . 2169343 . etal.
• Millake DB, Blanchard AD, Patel B, Critchley DR . The cDNA sequence of a human placental alpha-actinin. . Nucleic Acids Res. . 17 . 16 . 6725 . 1989 . 2780298 . 318366 . 10.1093/nar/17.16.6725 .
• Dubernard V, Faucher D, Launay JM, Legrand C . Identification of the cytoskeletal protein alpha-actinin as a platelet thrombospondin-binding protein. . FEBS Lett. . 364 . 2 . 109–14 . 1995 . 7750553 . 10.1016/0014-5793(95)00362-D . 44955001 . free .
• Knudsen KA, Soler AP, Johnson KR, Wheelock MJ . Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. . J. Cell Biol. . 130 . 1 . 67–77 . 1995 . 7790378 . 2120515 . 10.1083/jcb.130.1.67 .
• Glück U, Ben-Ze'ev A . Modulation of alpha-actinin levels affects cell motility and confers tumorigenicity on 3T3 cells. . J. Cell Sci. . 107 . 7. 1773–82 . 1995 . 10.1242/jcs.107.7.1773 . 7983147 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Egerton M, Moritz RL, Druker B . Identification of the 70kD heat shock cognate protein (Hsc70) and alpha-actinin-1 as novel phosphotyrosine-containing proteins in T lymphocytes. . Biochem. Biophys. Res. Commun. . 224 . 3 . 666–74 . 1996 . 8713105 . 10.1006/bbrc.1996.1082 . etal.
• Mukai H, Toshimori M, Shibata H . Interaction of PKN with alpha-actinin. . J. Biol. Chem. . 272 . 8 . 4740–6 . 1997 . 9030526 . 10.1074/jbc.272.8.4740 . etal. free .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 . etal.
• Hazan RB, Norton L . The epidermal growth factor receptor modulates the interaction of E-cadherin with the actin cytoskeleton. . J. Biol. Chem. . 273 . 15 . 9078–84 . 1998 . 9535896 . 10.1074/jbc.273.15.9078 . free .
• Bunn RC, Jensen MA, Reed BC . Protein interactions with the glucose transporter binding protein GLUT1CBP that provide a link between GLUT1 and the cytoskeleton. . Mol. Biol. Cell . 10 . 4 . 819–32 . 1999 . 10198040 . 25204 . 10.1091/mbc.10.4.819.
• Reinhard M, Zumbrunn J, Jaquemar D . An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment. . J. Biol. Chem. . 274 . 19 . 13410–8 . 1999 . 10224105 . 10.1074/jbc.274.19.13410 . etal. free .

External links

• Actinin, alpha 1 Info with links in the Cell Migration Gateway

Notes and References

1. Youssoufian H, McAfee M, Kwiatkowski DJ . Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene . Am J Hum Genet . 47 . 1 . 62–72 . Jul 1990 . 2349951 . 1683765 .
2. Web site: ACTN2 - Alpha-actinin-2 - Homo sapiens (Human) - ACTN2 gene & protein . www.uniprot.org . 5 December 2021 . en.
3. Web site: Entrez Gene: ACTN1 actinin, alpha 1.
4. Dhavan R, Greer PL, Morabito MA, Orlando LR, Tsai LH . The cyclin-dependent kinase 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent manner . J. Neurosci. . 22 . 18 . 7879–91 . September 2002 . 12223541 . 10.1523/JNEUROSCI.22-18-07879.2002. 6758084 .
5. Gonzalez AM, Otey C, Edlund M, Jones JC . Interactions of a hemidesmosome component and actinin family members . J. Cell Sci. . 114 . Pt 23 . 4197–206 . December 2001 . 10.1242/jcs.114.23.4197 . 11739652 .
6. Bunn RC, Jensen MA, Reed BC . Protein interactions with the glucose transporter binding protein GLUT1CBP that provide a link between GLUT1 and the cytoskeleton . Mol. Biol. Cell . 10 . 4 . 819–32 . April 1999 . 10198040 . 25204 . 10.1091/mbc.10.4.819.
7. Vallenius T, Luukko K, Mäkelä TP . CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 . J. Biol. Chem. . 275 . 15 . 11100–5 . April 2000 . 10753915 . 10.1074/jbc.275.15.11100 . free .
8. Bauer K, Kratzer M, Otte M, de Quintana KL, Hagmann J, Arnold GJ, Eckerskorn C, Lottspeich F, Siess W . Human CLP36, a PDZ-domain and LIM-domain protein, binds to alpha-actinin-1 and associates with actin filaments and stress fibers in activated platelets and endothelial cells . Blood . 96 . 13 . 4236–45 . December 2000 . 10.1182/blood.V96.13.4236 . 11110697 .
9. Feng S, Reséndiz JC, Christodoulides N, Lu X, Arboleda D, Berndt MC, Kroll MH . Pathological shear stress stimulates the tyrosine phosphorylation of alpha-actinin associated with the glycoprotein Ib-IX complex . Biochemistry . 41 . 4 . 1100–8 . January 2002 . 11802708 . 10.1021/bi0156005 .
10. Asada M, Irie K, Morimoto K, Yamada A, Ikeda W, Takeuchi M, Takai Y . ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions . J. Biol. Chem. . 278 . 6 . 4103–11 . February 2003 . 12446711 . 10.1074/jbc.M209832200 . free .
11. Reinhard M, Zumbrunn J, Jaquemar D, Kuhn M, Walter U, Trueb B . An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment . J. Biol. Chem. . 274 . 19 . 13410–8 . May 1999 . 10224105 . 10.1074/jbc.274.19.13410 . free .
12. Li B, Trueb B . Analysis of the alpha-actinin/zyxin interaction . J. Biol. Chem. . 276 . 36 . 33328–35 . September 2001 . 11423549 . 10.1074/jbc.M100789200 . free.
13. Besco JA, Hooft van Huijsduijnen R, Frostholm A, Rotter A. Intracellular substrates of brain-enriched receptor protein tyrosine phosphatase rho (RPTPrho/PTPRT). . Brain Res . 2006 . 1116 . 1 . 50–7 . 16973135 . 10.1016/j.brainres.2006.07.122 . 23343123 .