Alcohol dehydrogenase (nicotinoprotein) explained

Alcohol dehydrogenase (nicotinoprotein)
Ec Number:	1.1.99.36

Alcohol dehydrogenase (nicotinoprotein) (NDMA-dependent alcohol dehydrogenase, nicotinoprotein alcohol dehydrogenase, np-ADH, ethanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase) is an enzyme with systematic name ethanol:acceptor oxidoreductase.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

ethanol + acceptor

\rightleftharpoons acetaldehyde + reduced acceptor

This enzyme contains Zn²⁺.

Notes and References

1. Van Ophem PW, Van Beeumen J, Duine JA . Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica . European Journal of Biochemistry . 212 . 3 . 819–26 . March 1993 . 8385013 . 10.1111/j.1432-1033.1993.tb17723.x . free .
2. Piersma SR, Visser AJ, de Vries S, Duine JA . Optical spectroscopy of nicotinoprotein alcohol dehydrogenase from Amycolatopsis methanolica: a comparison with horse liver alcohol dehydrogenase and UDP-galactose epimerase . Biochemistry . 37 . 9 . 3068–77 . March 1998 . 9485460 . 10.1021/bi972115u .
3. Schenkels P, Duine JA . Nicotinoprotein (NADH-containing) alcohol dehydrogenase from Rhodococcus erythropolis DSM 1069: an efficient catalyst for coenzyme-independent oxidation of a broad spectrum of alcohols and the interconversion of alcohols and aldehydes . Microbiology . 146 (Pt 4) . 4 . 775–85 . April 2000 . 10784035 . 10.1099/00221287-146-4-775 . free .
4. Piersma SR, Norin A, de Vries S, Jörnvall H, Duine JA . Inhibition of nicotinoprotein (NAD+-containing) alcohol dehydrogenase by trans-4-(N,N-dimethylamino)-cinnamaldehyde binding to the active site . Journal of Protein Chemistry . 22 . 5 . 457–61 . July 2003 . 14690248 . 10.1023/b:jopc.0000005461.53788.ee .
5. Norin A, Piersma SR, Duine JA, Jörnvall H . Nicotinoprotein (NAD+ -containing) alcohol dehydrogenase: structural relationships and functional interpretations . Cellular and Molecular Life Sciences . 60 . 5 . 999–1006 . May 2003 . 12827287 . 10.1007/s00018-003-3105-9 . 11138879 .