Alcohol dehydrogenase (azurin) explained

Alcohol dehydrogenase (azurin)
Ec Number:	1.1.9.1

Alcohol dehydrogenase (azurin) (type II quinoprotein alcohol dehydrogenase, quinohaemoprotein ethanol dehydrogenase, QHEDH, ADHIIB) is an enzyme with systematic name alcohol:azurin oxidoreductase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

\rightleftharpoons aldehyde + reduced azurin

This enzyme is a periplasmic PQQ-containing quinohemoprotein.

Notes and References

1. Groen BW, van Kleef MA, Duine JA . Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni . The Biochemical Journal . 234 . 3 . 611–5 . March 1986 . 3521592 . 1146615 . 10.1042/bj2340611 .
2. de Jong GA, Caldeira J, Sun J, Jongejan JA, de Vries S, Loehr TM, Moura I, Moura JJ, Duine JA . Characterization of the interaction between PQQ and heme c in the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni . Biochemistry . 34 . 29 . 9451–8 . July 1995 . 7626615 . 10.1021/bi00029a021 .
3. Toyama H, Fujii A, Matsushita K, Shinagawa E, Ameyama M, Adachi O . Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonas putida is grown on different alcohols . Journal of Bacteriology . 177 . 9 . 2442–50 . May 1995 . 7730276 . 176903 . 10.1128/jb.177.9.2442-2450.1995 .
4. Matsushita K, Yamashita T, Aoki N, Toyama H, Adachi O . Electron transfer from quinohemoprotein alcohol dehydrogenase to blue copper protein azurin in the alcohol oxidase respiratory chain of Pseudomonas putida HK5 . Biochemistry . 38 . 19 . 6111–8 . May 1999 . 10320337 . 10.1021/bi990121f .
5. Chen ZW, Matsushita K, Yamashita T, Fujii TA, Toyama H, Adachi O, Bellamy HD, Mathews FS . Structure at 1.9 A resolution of a quinohemoprotein alcohol dehydrogenase from Pseudomonas putida HK5 . Structure . 10 . 6 . 837–49 . June 2002 . 12057198 . 10.1016/s0969-2126(02)00774-8 . free .
6. Oubrie A, Rozeboom HJ, Kalk KH, Huizinga EG, Dijkstra BW . Crystal structure of quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni: structural basis for substrate oxidation and electron transfer . The Journal of Biological Chemistry . 277 . 5 . 3727–32 . February 2002 . 11714714 . 10.1074/jbc.M109403200 . free .