Aerobactin synthase explained

aerobactin synthase
Ec Number:	6.3.2.39
Cas Number:	94047-30-0
Go Code:	0050565

In enzymology, an aerobactin synthase is an enzyme that catalyzes the chemical reaction

4 ATP + citrate + 2 N⁶-acetyl-N⁶-hydroxy-L-lysine + 2 H₂O

\rightleftharpoons 4 ADP + 4 phosphate + aerobactin

The 4 substrates of this enzyme are ATP, citrate, N6-acetyl-N6-hydroxy-L-lysine, and H₂O, whereas its 3 products are ADP, phosphate, and aerobactin.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is citrate:N⁶-acetyl-N⁶-hydroxy-L-lysine ligase (ADP-forming). This enzyme is also called citrate:6-N-acetyl-6-N-hydroxy-L-lysine ligase (ADP-forming). This enzyme participates in lysine degradation.

References

• Appanna DL, Grundy BJ, Szczepan EW, Viswanatha T . 1984 . Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1 . Biochim. Biophys. Acta . 801 . 437 - 443 . 10.1016/0304-4165(84)90150-8.
• Gibson F, Magrath DI . 1969 . The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I . Biochim. Biophys. Acta . 192 . 175 - 84 . 4313071 . 2 . 10.1016/0304-4165(69)90353-5.
• Maurer PJ, Miller M . 1982 . Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine . J. Am. Chem. Soc. . 104 . 3096 - 3101 . 10.1021/ja00375a025 . 11 .
• de Lorenzo V, Bindereif A, Paw BH, Neilands JB . 1986 . Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12 . J. Bacteriol. . 165 . 570 - 8 . 2935523 . 2 . 214457 . 10.1128/jb.165.2.570-578.1986 .
• Challis GL . 2005 . A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases . ChemBioChem . 6 . 601 - 11 . 15719346 . 10.1002/cbic.200400283 . 4 .