Adenosylcobyric acid synthase (glutamine-hydrolysing) explained

Adenosylcobyric acid synthase (glutamine-hydrolyzing)
Ec Number:	6.3.5.10
Go Code:	0051921

In enzymology, an adenosylcobyric acid synthase (glutamine-hydrolysing) is an enzyme that catalyzes the chemical reaction

4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H₂O

\rightleftharpoons 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

The four substrates of this enzyme are ATP, adenosylcobyrinic acid a,c-diamide, L-glutamine, and H₂O; its four products are ADP, phosphate, adenosylcobyric acid, and L-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor (Glutamine amidotransferases). The systematic name of this enzyme class is adenosylcobyrinic-acid-a,c-diamide:L-glutamine amido-ligase (ADP-forming). This enzyme is part of the biosynthetic pathway to cobalamin (vitamin B₁₂) in bacteria.

See also

• Cobalamin biosynthesis

References

• Blanche F, Couder M, Debussche L, Thibaut D, Cameron B, Crouzet J . 1991 . Biosynthesis of vitamin B12: stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide is catalyzed by one enzyme in Pseudomonas denitrificans . J. Bacteriol. . 173 . 6046 - 51 . 1917839 . 19 . 208350 .
• Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC . 2002 . The biosynthesis of adenosylcobalamin (vitamin B12) . Nat. Prod. Rep. . 19 . 390 - 412 . 12195810 . 10.1039/b108967f . 4 .