Adenosylcobinamide kinase explained

adenosylcobinamide kinase
Ec Number:	2.7.1.156
Go Code:	0043752

In enzymology, an adenosylcobinamide kinase is an enzyme that catalyzes the chemical reaction

RTP + adenosylcobinamide

\rightleftharpoons adenosylcobinamide phosphate + RDP

Thus, the two substrates of this enzyme are RTP and adenosylcobinamide, whereas its two products are adenosylcobinamide phosphate and RDP.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is RTP:adenosylcobinamide phosphotransferase. Other names in common use include CobU, adenosylcobinamide kinase/adenosylcobinamide-phosphate, guanylyltransferase, and AdoCbi kinase/AdoCbi-phosphate guanylyltransferase. This enzyme participates in porphyrin and chlorophyll metabolism.

References

• O'Toole GA, Escalante-Semerena JC . 1995 . Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate . J. Biol. Chem. . 270 . 23560 - 9 . 7559521 . 10.1074/jbc.270.40.23560 . 40 . free .
• Thompson TB, Thomas MG, Escalante-Semerena JC, Rayment I . 1998 . Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 A resolution . Biochemistry . 37 . 7686 - 95 . 9601028 . 10.1021/bi973178f . 21 . 10.1.1.565.1238 .
• Thompson TB, Thomas MG, Escalante-Semerena JC, Rayment I . 1999 . Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site . Biochemistry . 38 . 12995 - 3005 . 10529169 . 10.1021/bi990910x . 40 .
• Thomas MG, Thompson TB, Rayment I, Escalante-Semerena JC . 2000 . Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation . J. Biol. Chem. . 275 . 27576 - 86 . 10869342 . 36 . 10.1074/jbc.M000977200 . free .
• Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC . 2002 . The biosynthesis of adenosylcobalamin (vitamin B12) . Nat. Prod. Rep. . 19 . 390 - 412 . 12195810 . 10.1039/b108967f . 4 .