(acyl-carrier-protein) S-malonyltransferase explained

Malonyl-CoA:ACP Transacylase
Ec Number:2.3.1.39
Cas Number:37257-17-3
Go Code:0004314

In enzymology, a [acyl-carrier-protein] S-malonyltransferase is an enzyme that catalyzes the chemical reaction

malonyl-CoA + acyl carrier protein CoA + malonyl-[acyl-carrier-protein]

Thus, the two substrates of this enzyme are malonyl-CoA and acyl carrier protein, whereas its two products are CoA and malonyl-acyl-carrier-protein. This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. This enzyme participates in fatty acid biosynthesis.

Nomenclature

The systematic name of this enzyme class is malonyl-CoA:[acyl-carrier-protein] S-malonyltransferase. Other names in common use include malonyl coenzyme A-acyl carrier protein transacylase,

Structure

Crystal Structures of FabD from E.coli [1] and Streptomyces coelicolor [2] are known and provide great insight into the catalytic mechanism of FabD. In E.Coli, FabD primarily involved in FAS pathway. However, in Streptomyces coelicolor, FabD is involved in FAS and polyketide synthase pathways. In both cases, the structures and active sites are very similar.

The protein has an α/β type architecture, but the fold is unique. the active site inferred from the location of the catalytic Ser92 contains a typical nucleophilic elbow as observed in α/ β hydrolases. Serine 92 is hydrogen bonded to His 201 in a fashion similar to various serine hydrolases. however, instead of the carboxylic acid typically found in catalytic triads, the main chain carbonyl of Gln 250 serves as a hydrogen bond acceptor in an interaction with His 201. Two other residues, Arg-117 and Glu-11 are also located in the active site, but their function is not clear.

Function

The fatty acid synthetic pathway is the principal route for the production of membrane phospholipid acyl chains in bacterial and plants.[3] The reaction sequence is carried out by a series of individual soluble proteins that are each encoded by a discrete gene, and the pathway intermediates are shuttled between the enzymes. Malony-CoA:ACP Transacylase (FabD) is one such individual soluble protein and catalyzes the following reaction:

malonyl-CoA + acyl carrier protein CoA + malonyl-[acyl-carrier-protein]

The transfer of malonate to acyl-carrier-protein (ACP) converts the acyl groups into thioester forms which are characteristic of acyl intermediates in fatty acid synthesis and which are strictly required for the condensation reactions catalyzed by β-ketoacyl-ACP synthetase.[4]

Mechanism

Malonyl-CoA:ACP Transacylase uses a ping-pong kinetic mechanism with a bound malonyl ester as the acyl intermediate attached to a serine residue residing within a GHSLG pentapeptide.[5] FabD first binds malonyl-CoA, the malonyl moiety is then transferred to the active siteSer 92, and CoA is released from the enzyme. ACP then binds and the malonyl moiety is transferred to the terminal sulfhydryl of the ACP prosthetic group. This reaction is readily reversible.[6]

Industrial relevance

Among all known metabolic pathways in living systems, fatty acid biosynthesis yields the most energy dense products.[7] As a result, microbial fatty acid derivatives are emerging as a promising renewable energy alternative to fossil fuel derived transportation fuels. Recently, Khosla et al. have devised a procedure to reconstitute E.Coli Fatty Acid Synthase using purified protein components (including FabD) and reported a detailed kinetic analysis of this in-vitro reconstituted system.[8] Their finding provide a new basis for assessing the scope and limitations of using E.Coli as a biocatalyst for the production of diesel fuels.

Clinical relevance

FabD as a target for Antibacterial Drug Discovery: An upcoming field

Fatty acid biosynthesis is carried out by the ubiquitous Fatty Acid Synthase.[9] Fatty acid synthase pathways are divided into two distinct molecular forms: Type I and Type II.[10] In Type I, Fatty Acid Synthase (found in humans and other mammals) is a single large polypeptide composed of several distinct domains.[11] On the other hand, each enzymatic activity (Condensation reaction, Reduction Reaction, Dehydration reaction) is found as a discrete protein in type II systems.[12] The difference in active site organization and predominance of type II FAS systems in bacteria make the enzymes of this pathway attractive targets for antibacterials.

FabD (Acyl-Carrier-Protein S-Malonyltransferase) is a reasonable target given that a high resolution crystal structure is available. However, no FabD inhibitors have been reported in the literature and review articles on this topic. The simple structure and acidity of malonate seem to permit few approaches to synthesizing derivatives (acting as potential inhibitors) that retain the character of the molecule.

A second approach for using FabD as a drug target is frequently identified in the literature: FabD can provide a useful tag for locating fab genes because FabD gene is usually adjacent to at least one other fab gene.[13] However (as of 2015), no potential drugs have attempted to exploit this feature.

Further reading

Notes and References

  1. Serre L, Verbree EC, Dauter Z, Stuitje AR, Derewenda ZS . The Escherichia coli malonyl-CoA:acyl carrier protein transacylase at 1.5-A resolution. Crystal structure of a fatty acid synthase component . The Journal of Biological Chemistry . 270 . 22 . 12961–4 . Jun 1995 . 7768883 . 10.1074/jbc.270.22.12961 . free .
  2. Keatinge-Clay AT, Shelat AA, Savage DF, Tsai SC, Miercke LJ, O'Connell JD, Khosla C, Stroud RM . Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA:ACP transacylase . Structure . 11 . 2 . 147–54 . Feb 2003 . 12575934 . 10.1016/s0969-2126(03)00004-2 . free .
  3. White SW, Zheng J, Zhang YM . The structural biology of type II fatty acid biosynthesis . Annual Review of Biochemistry . 74 . 1 . 791–831 . Jun 2005 . 15952903 . 10.1146/annurev.biochem.74.082803.133524 .
  4. Ruch FE, Vagelos PR . The isolation and general properties of Escherichia coli malonyl coenzyme A-acyl carrier protein transacylase . The Journal of Biological Chemistry . 248 . 23 . 8086–94 . Dec 1973 . 10.1016/S0021-9258(19)43197-9 . 4584822 . free .
  5. Ruch FE, Vagelos PR . The isolation and general properties of Escherichia coli malonyl coenzyme A-acyl carrier protein transacylase . The Journal of Biological Chemistry . 248 . 23 . 8086–94 . Dec 1973 . 10.1016/S0021-9258(19)43197-9 . 4584822 . free .
  6. Oefner C, Schulz H, D'Arcy A, Dale GE . Mapping the active site of Escherichia coli malonyl-CoA-acyl carrier protein transacylase (FabD) by protein crystallography . Acta Crystallographica Section D . 62 . Pt 6 . 613–618 . Jun 2006 . 16699188 . 10.1107/S0907444906009474 .
  7. Liu T, Khosla C . Genetic engineering of Escherichia coli for biofuel production . Annual Review of Genetics . 44 . 53–69 . 2010 . 20822440 . 10.1146/annurev-genet-102209-163440 .
  8. Yu X, Liu T, Zhu F, Khosla C . In vitro reconstitution and steady-state analysis of the fatty acid synthase from Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America . 108 . 46 . 18643–8 . Nov 2011 . 22042840 . 10.1073/pnas.1110852108 . 3219124. 2011PNAS..10818643Y . free .
  9. Payne DJ, Warren PV, Holmes DJ, Ji Y, Lonsdale JT . Bacterial fatty-acid biosynthesis: a genomics-driven target for antibacterial drug discovery . Drug Discovery Today . 6 . 10 . 537–544 . May 2001 . 11369293 . 10.1016/S1359-6446(01)01774-3 .
  10. Rock CO, Cronan JE . Escherichia coli as a model for the regulation of dissociable (type II) fatty acid biosynthesis . Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism . 1302 . 1 . 1–16 . Jul 1996 . 8695652 . 10.1016/0005-2760(96)00056-2 .
  11. Chirala SS, Huang WY, Jayakumar A, Sakai K, Wakil SJ . Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities . Proceedings of the National Academy of Sciences of the United States of America . 94 . 11 . 5588–93 . May 1997 . 9159116 . 10.1073/pnas.94.11.5588 . 20822. 1997PNAS...94.5588C . free .
  12. Tsay JT, Oh W, Larson TJ, Jackowski S, Rock CO . Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12 . The Journal of Biological Chemistry . 267 . 10 . 6807–14 . Apr 1992 . 10.1016/S0021-9258(19)50498-7 . 1551888 . free .
  13. Campbell JW, Cronan JE . Bacterial fatty acid biosynthesis: targets for antibacterial drug discovery . Annual Review of Microbiology . 55 . 305–32 . 2001 . 11544358 . 10.1146/annurev.micro.55.1.305 .

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "(acyl-carrier-protein) S-malonyltransferase".

Except where otherwise indicated, Everything.Explained.Today is © Copyright 2009-2024, A B Cryer, All Rights Reserved. Cookie policy.