| Partial thromboplastin time | |
| Synonyms: | Activated partial thromboplastin time; Activated partial prothrombin time; Activated partial thrombin time |
The partial thromboplastin time (PTT), also known as the activated partial thromboplastin time (aPTT or APTT), is a blood test that characterizes coagulation of the blood. A historical name for this measure is the kaolin-cephalin clotting time (KCCT),[1] reflecting kaolin and cephalin as materials historically used in the test. Apart from detecting abnormalities in blood clotting,[2] partial thromboplastin time is also used to monitor the treatment effect of heparin, a widely prescribed drug that reduces blood's tendency to clot.
The PTT measures the overall speed at which blood clots form by means of two consecutive series of biochemical reactions known as the intrinsic pathway and common pathway of coagulation. The PTT indirectly measures action of the following coagulation factors: I (fibrinogen), II (prothrombin), V (proaccelerin), VIII (anti-hemophilic factor), X (Stuart–Prower factor), XI (plasma thromboplastin antecedent), and XII (Hageman factor).
The PTT is often used in conjunction with another measure of how quickly blood clotting takes place called the prothrombin time (PT). The PT measures the speed of clotting by means of the extrinsic pathway and common pathway.
Partial thromboplastin time is typically analyzed by a medical technologist or a laboratory technician on an automated instrument at 37 °C (as a nominal approximation of normal human body temperature). The test is termed "partial" due to the absence of tissue factor from the reaction mixture.
The typical reference range is between 25 seconds and 33 s (depending on laboratory). Longer times of up to 50 s do apply to infants. Shortening of the PTT is considered to have little clinical relevance, but some research indicates that it might increase risk of thromboembolism.[3] Normal PTT requires the presence of the following coagulation factors: I, II, V, VIII, IX, X, XI and XII. Notably, deficiencies in factors VII or XIII will not be detected with the PTT test.
Prolonged aPTT may indicate:[4]
To distinguish the above causes, mixing tests are performed, in which the patient's plasma is mixed (initially at a 50:50 dilution) with normal plasma. If the abnormality does not disappear, the sample is said to contain an "inhibitor" (either heparin, antiphospholipid antibodies or coagulation factor specific inhibitors), while if it does disappear a factor deficiency is more likely. Deficiencies of factors VIII, IX, XI and XII and rarely von Willebrand factor (if causing a low factor VIII level) may lead to a prolonged aPTT correcting on mixing studies.
The aPTT is usually normal in pregnancy but tends to slightly decrease in late pregnancy.[5]
See also: Activated protein C resistance test.
The aPTT-based activated protein C (APC) resistance test is used in the diagnosis of APC resistance (APCR).[6] It involves a modified aPTT test performed in the presence and absence of APC.[7] The ratio of these aPTT values is calculated and is called the APC sensitivity ratio (APCsr) or simply APC ratio (APCr). This ratio is inversely related to the degree of APC resistance.[8] The aPTT-based APC resistance test was developed in 1993.
The PTT was first described in 1953 by researchers at the University of North Carolina at Chapel Hill.[9]