A-1,6-mannosyl-glycoprotein 6-b-N-acetylglucosaminyltransferase explained

Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase
Ec Number:	2.4.1.155
Cas Number:	83588-90-3

Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase V, alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-alpha-mannoside beta1->6-acetylglucosaminyltransferase, UDP-N-acetylglucosamine:alpha-mannoside-beta1,6 N-acetylglucosaminyltransferase, alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetylglucosaminyltransferase, GnTV) is an enzyme with systematic name UDP-N-acetyl-D-glucosamine:6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase.^{[1] [2]} This enzyme catalyses the following chemical reaction

UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R

\rightleftharpoons UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R

R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor.

Notes and References

1. Cummings RD, Trowbridge IS, Kornfeld S . A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase . The Journal of Biological Chemistry . 257 . 22 . 13421–7 . November 1982 . 6216250 .
2. Hindsgaul O, Tahir SH, Srivastava OP, Pierce M . The trisaccharide beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp, as its 8-methoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V . Carbohydrate Research . 173 . 2 . 263–72 . March 1988 . 2834054 . 10.1016/s0008-6215(00)90822-x .