A-1,3-mannosyl-glycoprotein 2-b-N-acetylglucosaminyltransferase explained

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Ec Number:	2.4.1.101
Cas Number:	102576-81-8

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase I, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I, uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase, UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, GnTI) is an enzyme with systematic name UDP-N-acetyl-D-glucosamine:3-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase.^{[1] [2] [3] [4] [5] [6] [7] [8]} This enzyme catalyses the following chemical reaction

UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R

\rightleftharpoons UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R

R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor.

Notes and References

1. Harpaz N, Schachter H . Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity . The Journal of Biological Chemistry . 255 . 10 . 4885–93 . May 1980 . 6445358 .
2. Mendicino J, Chandrasekaran EV, Anumula KR, Davila M . Isolation and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa . Biochemistry . 20 . 4 . 967–76 . February 1981 . 6452163 . 10.1021/bi00507a050 .
3. Miyagi T, Tsuiki S . Studies on UDP-N-acetylglucosamine : alpha-mannoside beta-N-acetylglucosaminyltransferase of rat liver and hepatomas . Biochimica et Biophysica Acta (BBA) - Enzymology . 661 . 1 . 148–57 . September 1981 . 6170335 . 10.1016/0005-2744(81)90094-2 .
4. Oppenheimer CL, Eckhardt AE, Hill RL . The nonidentity of porcine N-acetylglucosaminyltransferases I and II . The Journal of Biological Chemistry . 256 . 22 . 11477–82 . November 1981 . 6457827 .
5. Oppenheimer CL, Hill RL . Purification and characterization of a rabbit liver alpha 1 goes to 3 mannoside beta 1 goes to 2 N-acetylglucosaminyltransferase . The Journal of Biological Chemistry . 256 . 2 . 799–804 . January 1981 . 6450208 .
6. Book: Schachter H, Narasimhan S, Gleeson P, Vella G . Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type . Methods in Enzymology . 98 . 98–134 . 1983 . 6366476 . 10.1016/0076-6879(83)98143-0 .
7. Vella GJ, Paulsen H, Schachter H . Control of glycoprotein synthesis. IX. A terminal Man alpha l-3Man beta 1- sequence in the substrate is the minimum requirement for UDP-N-acetyl-D-glucosamine: alpha-D-mannoside (GlcNAc to Man alpha 1-3) beta 2-N-acetylglucosaminyltransferase I . Canadian Journal of Biochemistry and Cell Biology . 62 . 6 . 409–17 . June 1984 . 6235906 . 10.1139/o84-056 .
8. Unligil UM, Zhou S, Yuwaraj S, Sarkar M, Schachter H, Rini JM . X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily . The EMBO Journal . 19 . 20 . 5269–80 . October 2000 . 11032794 . 314010 . 10.1093/emboj/19.20.5269 .