ZWINT explained

ZW10 interactor (Zwint-1) is a protein that in humans is encoded by the ZWINT gene.^[1]

Function

Zwint-1 is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Interactions

ZWINT has been shown to interact with MIS12.^{[2] [3]} ZWINT has also been shown to interact with RAB3C^[4]

References

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . Jan 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Research . 6 . 9 . 791–806 . Sep 1996 . 8889548 . 10.1101/gr.6.9.791 . free .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . Oct 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Starr DA, Saffery R, Li Z, Simpson AE, Choo KH, Yen TJ, Goldberg ML . HZwint-1, a novel human kinetochore component that interacts with HZW10 . Journal of Cell Science . 113 (Pt 11) . 11 . 1939–50 . Jun 2000 . 10.1242/jcs.113.11.1939 . 10806105 .
• Musio A, Mariani T, Montagna C, Zambroni D, Ascoli C, Ried T, Vezzoni P . Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes . Gene . 331 . 33–40 . Apr 2004 . 15094189 . 10.1016/j.gene.2004.01.028 .
• Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A . A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension . Genes & Development . 18 . 18 . 2255–68 . Sep 2004 . 15371340 . 517519 . 10.1101/gad.1234104 .
• Wang H, Hu X, Ding X, Dou Z, Yang Z, Shaw AW, Teng M, Cleveland DW, Goldberg ML, Niu L, Yao X . Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling . The Journal of Biological Chemistry . 279 . 52 . 54590–8 . Dec 2004 . 15485811 . 10.1074/jbc.M407588200 . free .
• Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M . A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1 . Nature Cell Biology . 6 . 11 . 1135–41 . Nov 2004 . 15502821 . 10.1038/ncb1187 . 39408000 .
• Le XF, Lammayot A, Gold D, Lu Y, Mao W, Chang T, Patel A, Mills GB, Bast RC . Genes affecting the cell cycle, growth, maintenance, and drug sensitivity are preferentially regulated by anti-HER2 antibody through phosphatidylinositol 3-kinase-AKT signaling . The Journal of Biological Chemistry . 280 . 3 . 2092–104 . Jan 2005 . 15504738 . 10.1074/jbc.M403080200 . free .
• Kops GJ, Kim Y, Weaver BA, Mao Y, McLeod I, Yates JR, Tagaya M, Cleveland DW . ZW10 links mitotic checkpoint signaling to the structural kinetochore . The Journal of Cell Biology . 169 . 1 . 49–60 . Apr 2005 . 15824131 . 1351127 . 10.1083/jcb.200411118 .
• Lin YT, Chen Y, Wu G, Lee WH . Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control . Oncogene . 25 . 52 . 6901–14 . Nov 2006 . 16732327 . 10.1038/sj.onc.1209687 . 11176319 .

Notes and References

1. Web site: Entrez Gene: ZWINT ZW10 interactor.
2. Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A . A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension . Genes & Development . 18 . 18 . 2255–68 . Sep 2004 . 15371340 . 517519 . 10.1101/gad.1234104 .
3. Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M . A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1 . Nature Cell Biology . 6 . 11 . 1135–41 . Nov 2004 . 15502821 . 10.1038/ncb1187 . 39408000 .
4. van Vlijmen T, Vleugel M, Evers M, Mohammed S, Wulf PS, Heck AJ, Hoogenraad CC, van der Sluijs P . Casper Hoogenraad . A unique residue in rab3c determines the interaction with novel binding protein Zwint-1 . FEBS Letters . 582 . 19 . 2838–42 . Aug 2008 . 18625232 . 10.1016/j.febslet.2008.07.012 . 30206840 . free .