Y187 Explained

Y187 is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain has been sold commercially by Clontech^[1] since at least 2000 and is used as a partner with strain AH109 in mating assays.^[2]

Genotype of Y187: MATα, ura3-52, his3-200, ade2-101, trp1-901, leu2-3, 112, gal4Δ, met–, gal80Δ, URA3::GAL1_UAS-GAL1_TATA-lacZ.^[3]

Notes and References

1. Fromont-Racine. Micheline . Rain, Jean-Christophe . Legrain, Pierre. Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens. Nature Genetics. 1997. 16. 3. 277–282. 10.1038/ng0797-277. 9207794. 32591856 .
2. Book: Guo, Deyin . Rajamäki, Minna-Liisa . Valkonen, Jari . Plant Virology Protocols . Protein–Protein Interactions: The Yeast Two-Hybrid System . Methods in Molecular Biology . 2008. 451 . 3. 421–439. 10.1007/978-1-59745-102-4_29. 18370272 . 978-1-58829-827-0 .
3. http://www.uni-jena.de/unijenamedia/Downloads/faculties/bio_pharm/ls_genetik/Teaching/Grundpraktikum/Y2Hscript_SS11.pdf{{dead link|date=July 2016 |bot=InternetArchiveBot |fix-attempted=yes }}