Xaa-Pro dipeptidyl-peptidase explained

Xaa-Pro dipeptidyl-peptidase
Ec Number:	3.4.14.11
Cas Number:	54249-88-6

Xaa-Pro dipeptidyl-peptidase (X-prolyl dipeptidyl aminopeptidase, PepX, X-prolyl dipeptidyl peptidase is an enzyme.^{[1] [2] [3] [4] [5]} It catalyses the following chemical reaction

Hydrolyses Xaa-Pro bonds to release unblocked, N-terminal dipeptides from substrates including Ala-Pro-p-nitroanilide and (sequentially) Tyr-Pro--Phe-Pro--Gly-Pro--Ile

The intracellular enzyme from Lactococcus lactis (190-kDa) is the type example of peptidase family S15.

Notes and References

1. Zevaco C, Monnet V, Gripon JC . Intracellular X-prolyl dipeptidyl peptidase from Lactococcus lactis spp. lactis: purification and properties . Journal of Applied Bacteriology . 1990 . 68 . 4 . 357–366 . 10.1111/j.1365-2672.1990.tb02886.x.
2. Meyer-Barton EC, Klein JR, Imam M, Plapp R . Cloning and sequence analysis of the X-prolyl-dipeptidyl-aminopeptidase gene (pepX) from Lactobacillus delbrückii ssp. lactis DSM7290 . Applied Microbiology and Biotechnology . 40 . 1 . 82–9 . October 1993 . 7765315 . 10.1007/bf00170433 .
3. Purification and characterization of X-prolyl dipeptidyl peptidase from Lactobacillus casei subsp. casei LLG . Habibi-Najafi MB, Lee BH . Appl. Microbiol. Biotechnol. . 1994 . 42 . 2–3 . 280–286 . 7765768 . 10.1007/s002530050250.
4. Chich JF, Gripon JC, Ribadeau-Dumas B . Preparation of bacterial X-prolyl dipeptidyl aminopeptidase and its stabilization by organic cosolvents . Analytical Biochemistry . 224 . 1 . 245–9 . January 1995 . 7710078 . 10.1006/abio.1995.1036 .
5. Chich JF, Chapot-Chartier MP, Ribadeau-Dumas B, Gripon JC . Identification of the active site serine of the X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis . FEBS Letters . 314 . 2 . 139–42 . December 1992 . 1459244 . 10.1016/0014-5793(92)80960-o . free .