The Wolstenholme Baronetcy, of London, was a title in the Baronetage of England. It was created on 10 January 1665 for John Wolstenholme, who had previously represented West Looe, Newport and Queenborough in Parliament. He had been heavily fined by the Parliamentarians for supporting the Royal cause during the Civil War. The third Baronet sat as Member of Parliament for Middlesex. The title became extinct on the death of the seventh Baronet in 1762.