| State: | WV |
| Type: | WV |
| Route: | 331 |
| Map Custom: | yes |
| Map Notes: | WV 331 highlighted in red |
| Length Mi: | 4.0 |
| Length Round: | 1 |
| Direction A: | West |
| Terminus A: | in Mount Alto |
| Direction B: | East |
| Terminus B: | in Cottageville |
| Counties: | Jackson |
| Previous Type: | WV |
| Previous Route: | 311 |
| Next Type: | WV |
| Next Route: | 338 |
West Virginia Route 331 (WV 331) is an east - west state highway located entirely in Jackson County, West Virginia. The western terminus of the route is at West Virginia Route 2 and West Virginia Route 62 in Mount Alto. The eastern terminus is at WV 62 near Cottageville.
WV 331 is a former alignment of U.S. Route 33, which was later moved to follow all of present WV 62 between Mason and Ripley.