Wayson stain explained

The Wayson stain is a basic fuchsin-methylene blue, ethyl alcohol-phenol microscopic staining procedure. It was originally a modified methylene blue stain used for diagnosing bubonic plague.[1] With this stain, Yersinia pestis appears purple with a characteristic safety-pin appearance,[2] which is due to the presence of a central vacuole.

Wayson stain is used along with the Giemsa and Wright's stains to rapidly detect potential biowarfare attacks.[3] It has also been investigated as a possible cheaper and faster way to detect melioidosis.[4] It is a useful alternative to the Gram or Loeffler's Methylene Blue stains, especially for detecting Yersinia enterocolitica which is often found in contaminated food.[5]

External links

Notes and References

  1. Meyer KF, Batchelder AP . 1926. A disease in wild rats caused by Pasteurella muricida, n. sp. J Infect Dis. 39. 386–412. 10.1093/infdis/39.5.386. 5.
  2. Inglesby TV . Plague as a biological weapon: medical and public health management. Working Group on Civilian Biodefense . JAMA . 283 . 17 . 2281–90 . May 2000 . 10807389 . 10.1001/jama.283.17.2281 . vanc . Dennis DT . Henderson DA . 3 . Bartlett . JG . Ascher . MS . Eitzen . E . Fine . AD . Friedlander . AM . Hauer . J.
  3. http://www.medscape.com/content/2001/00/42/14/421420/421420_tab.html Medscape.com Clues to the Specific Etiologic Agent Used in a Biological Attack
  4. Sheridan EA, Ramsay AR, Short JM, Stepniewska K, Wuthiekanun V, Simpson AJ . Evaluation of the Wayson Stain for the Rapid Diagnosis of Melioidosis . J. Clin. Microbiol. . 45 . 5 . 1669–70 . May 2007 . 17360835 . 1865910 . 10.1128/JCM.00396-07 .
  5. http://www.clpmag.com/issues/articles/2001-08_22.asp CLP Magazine Aug 2001

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